OPTIZYME™ RsaI, Fisher BioReagents™

Description

5'...G T^A C...3'
3'...C A^T G...5'

Supplied with: 10X OPTIZYME Buffer 4
Conditions for 100% Activity:

• 1X OPTIZYME Buffer 4: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/ml BSA
• Incubate at 37°C

Enzyme Activity in OPTIZYME buffers:

• Buffer 1: 50 - 100%
• Buffer 2: 20 - 50%
• Buffer 3: 0 - 20%
• Buffer 4: 100%
• Buffer 5: 0 - 20%

Storage Buffer:

• 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerol

Ligation and Re-cleavage:

• More than 95% of the DNA fragments can be ligated and re-cut after a 50-fold over-digestion with RsaI

Methylation Effects:

Dam: Never overlaps - no effect

Dcm: Never overlaps - no effect

CpG: May overlap - cleavage impaired

• 5'...GTAm5C G...3'

3'...CAT Gm5C...5'

(Cleavage is impaired)

• 5'...m5C GTAm5C G...3'

3'... Gm5CAT Gm5C...5'

(Cleavage is blocked)

EcoKI: Never overlaps - no effect

EcoBI: Never overlaps - no effect

Specifications

• Concentration: 10 U/μL
• Components: >50% Glycerin, 25 to 50% Water
• Incubator Temperature: 37°C
• pH: 7.4
• For Use With (Application): >90% of DNA fragments can be ligated and re-cut after 10-fold over-digestion with Rsa I
• Storage Buffer: 10mM Tris HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA, 50% Glycerol
• Content And Storage: Keep container tightly closed
• Quantity: 1000 U
• Cut Site: GT.AC
• Product Type: RsaI
• Form: Liquid

Safety and Handling

SDS