Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Thermo Scientific™ Membrane Clostridium Perfringens (m-CP) Agar Base (Dehydrated)

Rapidly isolate and presumptively identify Clostridium perfringens from water samples with this selective and chromogenic medium

Manufacturer:  Thermo Scientific™ CM0992B

Catalog No. OXCM0992B


Add to cart

Description

Description

Rapidly isolate and presumptively identify Clostridium perfringens from water samples with selective and chromogenic Thermo Scientific™ Oxoid™ Membrane Clostridium Perfringens (m-CP) Agar Base (Dehydrated) when used with m-CP Selective Supplement. The medium was first described by Bisson and Cabelli1 for the rapid quantitation of Clostridium perfringens from water samples.

The lack of b-D-glucosidase activity fermentation of sucrose and production of acid phosphatase differentiate presumptive Clostridium perfringens colonies from other Clostridium spp on the medium.

Use Membrane Clostridium Perfringens (m-CP) Agar Base for rapid isolation and presumptive identification of Clostridium perfringens from water samples.

  • Selective: D-cycloserine, polymyxin B and recommended incubation at 44°C inhibit the growth of background flora such as Gram-negative organisms and staphylococci
  • Chromogenic: Clostridium perfringens form characteristic yellow, opaque colonies while most other Clostridium appear as either purple colonies or blue/green colonies.
  • Recommended in European Council Directive 98/83/EC for testing the quality of water intended for human consumption2.
  • Shown to give better recovery of Clostridium perfringens from water and sewage samples than the Bonde pour tube method1.

In m-CP Medium lack of b-D-glucosidase activity (an enzyme involved in cellobiose fermentation), fermentation of sucrose and production of acid phosphatase are used to differentiate presumptive Clostridium perfringens colonies from other Clostridium spp.

Lack of b-D glucosidase activity means that Clostridium perfringens does not cleave the chromogen, indoxyl b-D glucoside, in the medium. Furthermore, as the organisms ferment the sucrose in the medium, reducing the pH, bromocresol purple changes from purple to yellow. This results in characteristic opaque yellow Clostridium perfringens colonies.

Most other Clostridium spp. will appear as either purple colonies, due to the lack of sucrose fermentation, or blue/green colonies where the organism is still cleaving Indoxyl b-D glucoside and also fermenting sucrose.

Presumptive positive Clostridium perfringens colonies can be further tested for acid phosphatase activity by exposure to ammonium hydroxide vapor for 20 to 30 seconds. Clostridium perfringens colonies turn pink or red as phenolphthalein diphosphate is cleaved by acid phosphatase. No color change will be seen with colonies of organisms that do not posses acid phosphatase. It is important this further test is carried out as there are a very small number of non-perfringens clostridia that produce yellow colonies. However, these colonies will remain yellow after exposure to ammonium hydroxide as they are acid phosphatase negative. 

Not all products are available for sale in all territories. Please inquire.

Remel™ and Oxoid™ products are now part of the Thermo Scientific brand

Specifications

Specifications

Membrane Clostridium perfringens (m-CP) Agar Base
For 7.0L medium
Media
Agar
500 g
Powder
SDS
Documents
Product Certifications
Promotions

Promotions

Provide Content Correction

We continue to work to improve your shopping experience and your feedback regarding this content is very important to us. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit

For Laboratory Use Only.