Thermo Scientific™ SIM Medium (Dehydrated)

Catalog No.	Quantity
OXCM0435B	500 g

Catalog No. OXCM0435B Supplier Thermo Scientific™ Supplier No. CM0435B

Description

Differentiate enteric bacteria on the basis of sulphide production, indole production and motility with this medium.

Differentiate enteric bacteria on the basis of sulphide production, indole production and motility with Thermo Scientific™ Oxoid™ SIM Medium (Dehydrated). The medium contains sodium thiosulphate and ferrous ammonium sulphate, which together act as indicators for the production of hydrogen sulphide. Tryptone incorporated into the medium is a tryptophan-rich peptone and is degraded by specific bacteria to produce indole. The use of 0.35% agar in the medium results in the production of a semi-solid medium, ideal for the examination of motility.

A motility-indole medium has been found to be helpful in the identification of the Enterobacteriaceae; e.g. in the differentiation of Klebsiella from Enterobacter and Serratia species¹. For convenience, these two important tests have been combined with sulphide-production in one tube. The production of hydrogen sulphide is a useful diagnostic test in the identification of enteric bacteria and is helpful in the differentiation between Salmonella and Shigella.

Typical reactions of organisms in SIM medium:

Organism	Motility	H2S	Indole
Escherichia coli ATCC® 25922	V	-	+
Proteus hauseri ATCC® 13315	+	+	+
Shigella sonnei ATCC® 25931	-	-	-

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Specifications

• Certifications/Compliance: Industrial Reference: FDA.
• Description: SIM Medium
• Form: Powder
• Product Type: Dehydrated Culture Media
• Quantity: 500 g
• Yield: For 16.7L medium

For Laboratory Use Only