Invitrogen™ GLP-1 Multispecies ELISA Kit

Description

Sandwich ELISA Kit

The Glucagon Like Peptide-1 (GLP-1) ELISA quantitates GLP-1 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant GLP-1 in Human, Mouse, Rat, Porcine, and Non-Human Primate samples.

Principle of the method
The GLP-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Target information
GCG is also known as GLP1, or Glucagon. Glucagon is a 29-amino acid pancreatic hormone that counteracts the glucose-lowering action of insulin by stimulating glycogenolysis and gluconeogenesis.It is mapped to 2q36-2q37. GLP1, also known as 7-37 for the codons of the preproglucagon molecule which encode it, renders pancreatic beta-cells 'glucose-competent' and may be useful in the treatment of noninsulin-dependent diabetes mellitus. Also, GLP1 is a potent insulin secretagogue.It plays a major role in the enteroinsular axis, accounting, for example, for the finding that plasma insulin levels accompanying oral intake of glucose are greater than those observed when glucose is given intravenously.

Specifications

• Assay Range: 0.8 to 50.0 pg/mL
• Assay Sensitivity: 0.32 pg/mL
• Conjugate: HRP
• Product Type: ELISA Kit
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Multi-species
• For Use With (Application): ELISA
• For Use With (Equipment): Colorimetric Microplate Reader
• Interassay CV: 3.8%
• Intraassay CV: 3.8%
• Kit Contents: Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, SAV-HRP, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Amniotic Fluid, 10 μL; Plasma, 10 μL; Serum, 10 μL; Supernatant, 10 μL
• Storage Requirements: 2°C to 8°C
• Target: Multi Species
• Test Time: 1 hr. 20 min.
• Total Assay Time: 3 hr. 30 min.

For Research Use Only