Invitrogen™ p23 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA528476

Catalog No. PIPA528476

Description

Recommended positive controls: 293T, A431, Jurkat, Raji, mouse brain. Predicted reactivity: Mouse (98%), Rat (98%), Xenopus laevis (84%), Chicken (96%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Steroid receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Prior to activation, steroid receptors associate with a number of different proteins in both a stable and transient fashion. Steroid receptor complex proteins include heat shock proteins (HSP70 and HSP90), immunosuppressant binding proteins called immunophilins (the FK506 binding proteins, FKBP52 & FKBP54 and the cyclosporin binding protein, CyP-40) and at least three other proteins termed p23, p60 and p48. p23 along with HSP70, HSP90 and p60, combine with progesterone receptor (PR) as members of a transient intermediate complex. Cloned human p23 encodes a protein of 160 amino acids that is highly conserved between species and shows no homology to previously identified proteins. p23 is a highly acidic phosphoprotein with an aspartic acid-rich C-terminal domain and multiple potential phosphorylation sites. In vitro studies have suggested that p23 binds to HSP90 and is necessary for the binding of HSP90 and CyP-40 to PR. While neither its exact function nor mechanism of action have been identified, p23 appears to be an important factor in PR function.

Specifications

• Antigen: p23
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Ptges3
• Gene Alias: 5730442A20Rik; Co chaperone p23; cPGES; cytosolic prostaglandin E synthase; cytosolic prostaglandin E2 synthase; Hsp90 co chaperone; Hsp90 co-chaperone; LOC100351480; P23; p23 cochaperone; progesterone receptor; progesterone receptor complex p23; Prostaglandin E synthase 3; prostaglandin E synthase 3 (cytosolic); prostaglandin-E synthase 3; Ptges; PTGES3; ptges3 {ECO:0000250; RGD1561913; sid 3177; Sid3177; Tebp; telomerase binding protein, p23; telomerase-binding protein p23; unactive progesterone receptor, 23 kD; UniProtKB:Q15185}
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 10728, 56351
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Concentration: 1 mg/mL
• Formulation: PBS with 1% BSA, 20% glycerol and 0.01% thimerosal; pH 7
• Gene Accession No.: Q15185, Q9R0Q7
• Gene Symbols: Ptges3
• Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 160 of Human p23.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG