Invitrogen™ Multispecies p70 S6 Kinase (Total) InstantOne™ ELISA Kit

Description

ELISA

The InstantOne™ ELISA is specifically engineered for accurate measurement of total human and mouse p70 S6K in cell lysates. Detection of rat p70 S6K is expected. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.

RPS6KB1 (p70S6K) is a serine/threonine kinase with 2 non-identical kinase catalytic domains which phosphorylates several residues of the S6 ribosomal protein. p70 S6 kinase is predominantly localized in the cytoplasm, and is essential in growth factors regulated cell proliferation, pathways involving cell motility, such as metastases, the immune response, and tissue repair. p70 S6 kinase acts downstream of phosphoinositide (PI) 3-kinase, and its main physiological target is the S6 ribosomal protein, which is involved in upregulation of protein synthesis. The kinase activity of p70 S6 kinase leads to an increase in protein synthesis and cell proliferation. Amplification of the region of DNA encoding p70 S6 kinase and overexpression are seen in some breast cancer cell lines.

Specifications

• Accession Number: P23443,Q8BSK8
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Cell Lysates
• Target Species: Mouse, Human
• For Use With (Application): ELISA
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 6198, 72508
• Gene Symbol: PS6K,S6K,S6K-beta-1,S6K1,STK14A,p70 S6KA,p70(S6K)-alpha,p70-S6K,p70-alpha
• Kit Contents: Capture Antibody, Detection Antibody Reagent, InstantOne™ Assay Plate, Cell Lysis Buffer, Enhancer Solution, Wash Buffer (10X), Substrate Reagent (TMB), Stop Solution, Positive Control Cell Lysate (lyophilized), Plate seal
• Label or Dye: HRP
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Cell Lysate 20 μL
• Storage Requirements: 2°C to 8°C
• Synonym: Ribosomal protein S6 kinase beta-1, RPS6KB1, STK14A
• Target: p70 S6 Kinase
• Test Time: 1 hrs 20 mins
• Total Assay Time: 1 hrs 10 mins

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov