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Invitrogen™ p70 S6 Kinase (Total/Phospho) Multispecies InstantOne™ ELISA Kit Non-distribution product as customer accommodation.

InstantOne ELISA

Manufacturer:  Invitrogen™ 858605311

Catalog No. 50-246-250


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Alert:

The Total/Phospho InstantOne ELISAs contain reagents for a total of 96 wells (24 wells total/72 wells phospho)
Please contact Technical Service for a lot specific COA


Includes: User Manual
InstantOne™ Assay Plate - 96 well (sealed)
Cell Lysis Buffer (5X)
Enhancer Solution
Wash Buffer (10X)
p70 S6K (Total) Capture Antibody Reagent
phospho-p70 S6K (Thr389) Capture Antibody Reagent
p70 S6K (Total) Detection Antibody Reagent
phospho-p70 S6K (Thr389) Detection Antibody Reagent
Positive Control Cell Lysate (lyophilized)
Detection Reagent (TMB Substrate)
Stop Solution
Plate Seal

Description

Description

The InstantOne™ ELISA is specifically engineered for accurate measurement of total and phosphorylated human and mouse p70 S6K in cell lysates. Detection of rat p70 S6K is expected.The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.

The p70 S6 kinase (p70 Ribosomal Protein S6 Kinase, p70S6K) is a 70 kDa member of the ribosomal S6 kinase (RSK) family of serine/threonine kinases. p70 S6 kinase is predominantly localized in the cytoplasm, and is essential in growth factors regulated cell proliferation, pathways involving cell motility, such as metastases, the immune response, and tissue repair. p70 S6 kinase acts downstream of phosphoinositide (PI) 3-kinase, and its main physiological target is the S6 ribosomal protein, which is involved in upregulation of protein synthesis. The kinase activity of p70 S6 kinase leads to an increase in protein synthesis and cell proliferation. Amplification of the region of DNA encoding p70 S6 kinase and overexpression are seen in some breast cancer cell lines.
Specifications

Specifications

P23443,Q8BSK8
ELISA
Capture Antibody
RUO
2°C to 8°C
1 hr. 10 min.
PS6K,S6K,S6K-beta-1,S6K1,STK14A,p70 S6KA,p70(S6K)-alpha,p70-S6K,p70-alpha
96 Tests
Cell Lysates
Colorimetric Microplate Reader
Biotin
6198, 72508
ELISA Kit
Cell Lysate,20 μL
1 hr. 20 min.
Human, Mouse
RPS6KB1, Ribosomal protein S6 kinase beta-1, S6K1
Human, Mouse
HRP
SDS
Documents
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