Invitrogen™ PARP1 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA3951

Catalog No. PA3951

Description

By Western blot, this antibody detects a 116 kDa protein representing PARP and the 85 kDa apoptosis-induced cleavage product of protease resembling ICE (prICE) and CPP32 from GH4C1 cell extract.

Poly ADP-Ribose Polymerase (PARP) uses nicotinamide adenine dinucleotide (oxidized form) NAD as a substrate to catalyse the transfer of ADP-ribose to a variety of nuclear protein acceptors. Proteolysis of PARP to its stable 85kDa fragment is an early marker of programmed cell death (apoptosis) and is mediated by the caspase CPP32 protein. Cleavage occurs between Adp216 and Gly217, a site in PARP conserved across species.

Specifications

• Antigen: PARP1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Parp1
• Gene Alias: 5830444G22Rik; ADP-ribosyltransferase (NAD+; ADP-ribosyltransferase (NAD+) poly (ADP-ribose) polymerase); ADP-ribosyltransferase (NAD+, poly (ADP-ribose) polymerase) 1; ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase); ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase) 1; ADP-ribosyltransferase 1; ADP-ribosyltransferase diphtheria toxin-like 1; ADP-ribosyltransferase NAD(+); Adprp; Adprt; ADPRT 1; Adprt1; AI893648; ARTD1; C80510; DNA ADP-ribosyltransferase PARP1; I79_014850; msPARP; NAD(+) ADP-ribosyltransferase 1; NAD(+) pADPRT-1; pADPRT-1; PARP; PARP1; PARP-1; poly (ADP-ribose) polymerase 1; poly (ADP-ribose) polymerase family, member 1; poly (ADP-ribose) polymerase); poly [ADP-ribose] polymerase 1; poly ADP-ribose polymerase; poly(ADP-ribose) polymerase; poly(ADP-ribose) polymerase 1; poly(ADP-ribose) polymerase PARP-1; poly(ADP-ribose) synthetase; poly(ADP-ribosyl)transferase; Poly[ADP-ribose] synthase 1; poly[ADP-ribose] synthetase 1; PPOL; Protein poly-ADP-ribosyltransferase PARP1; sPARP-1; synthetase fragment (AA 67); Unknown (protein for IMAGE:8023736); unnamed protein product
• Host Species: Rabbit
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat, Bovine
• Product Type: Antibody
• Isotype: IgG

• Applications: ELISA, Western Blot
• Concentration: Conc. Not Determined
• Formulation: whole serum with 0.05% sodium azide
• Gene Accession No.: P09874, P11103, P18493, P27008
• Gene Symbols: Parp1
• Immunogen: Synthetic peptide corresponding to aa 509-524 of bovine PARP auto modification domain.
• Regulatory Status: RUO
• Gene ID (Entrez): 11545, 142, 25591, 286764
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid