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ATCC pcDNA3L-His-CAMYEL plasmid in Escherichia coli - Mammalian expression vector encoding CAMYEL BRET cAMP sensor
Supplier: ATCC MBA277
pcDNA3L-His-CAMYEL plasmid is a mammalian expression vector encoding the CAMYEL cAMP sensor (YFP-Epac-RLuc) used for BRET-based monitoring of intracellular cAMP dynamics in cell signaling studies. The plasmid is maintained in Escherichia coli and provided for molecular biology workflows involving BRET assays.
- Encodes CAMYEL BRET cAMP sensor for real-time cAMP monitoring.
- Designed for expression in mammalian cells to support signaling studies.
- Maintained in Escherichia coli Stbl2 for plasmid propagation.
- Contains neomycin and ampicillin selection markers for selection in eukaryotic and bacterial systems.
- Construct size approximately 9.38 kb.
- Sequence data is provided to support experimental design and cloning.
- Requires long-term storage at -80°C to preserve construct integrity.
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