Promega pCI and pCI-neo Mammalian Expression Vectors

Description

Contains the CMV immediate-early enhancer/promoter region and is designed to promote constitutive expression of cloned DNA inserts in mammalian cells.

pCI Mammalian Expression Vector

• Designed to promote constitutive expression of cloned DNA inserts in mammalian cells
• Contains the human cytomegalovirus (CMV) major immediate-early gene enhancer/promoter region†
• Can be used for both transient and stable expression of genes
• Note: For stable expression, the pCI Vector must also be transfected with an expression vector containing a selectable gene for mammalian cells

pCI-neo Mammalian Expression Vector

• Similar to pCI Mammalian Expression Vector
• Also contains the neomycin phosphotransferase gene, a selectable marker for mammalian cells
• Can be used for transient expression or for stable expression by selecting transfected cells with the antibiotic G-418

Both Vectors

• Can also be used to synthesize transcripts in vitro using the T7 RNA polymerase promoter or generate single-stranded DNA in E. coli using the f1 origin of replication

Specifications

• Promoter: CMV
• Storage Buffer: TE Buffer (pH 8)
• Content And Storage: -30°C to -10°C
• Quantity: 20 μg
• Vector: pCI vector
• For Use With (Application): Promote constitutive expression of cloned DNA inserts in Mammalian cells, can also be used to synthesize transcripts in-vitro using the T7 RNA Polymerase promoter or generate single-stranded DNA in E. coli using the f1 origin of replication