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Promega pCI and pCI-neo Mammalian Expression Vectors
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Vector:
pCI vector
Description
pCI Mammalian Expression Vector
- Designed to promote constitutive expression of cloned DNA inserts in mammalian cells
- Contains the human cytomegalovirus (CMV) major immediate-early gene enhancer/promoter region†
- Can be used for both transient and stable expression of genes
- Note: For stable expression, the pCI Vector must also be transfected with an expression vector containing a selectable gene for mammalian cells
- Similar to pCI Mammalian Expression Vector
- Also contains the neomycin phosphotransferase gene, a selectable marker for mammalian cells
- Can be used for transient expression or for stable expression by selecting transfected cells with the antibiotic G-418
- Can also be used to synthesize transcripts in vitro using the T7 RNA polymerase promoter or generate single-stranded DNA in E. coli using the f1 origin of replication
Specifications
Specifications
Promoter | CMV |
Storage Buffer | TE Buffer (pH 8) |
Content And Storage | -30°C to -10°C |
Quantity | 20 μg |
Vector | pCI vector |
For Use With (Application) | Promote constitutive expression of cloned DNA inserts in Mammalian cells, can also be used to synthesize transcripts in-vitro using the T7 RNA Polymerase promoter or generate single-stranded DNA in E. coli using the f1 origin of replication |
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