Novus Biologicals™ PD-L1 Antibody Pair [HRP]

Description

Mouse Monoclonal Antibody Pair

This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human PD-L1.

Specifications

• Assay Range: 6.25 pg/mL
• Assay Sensitivity: 6.25-400 pg/mL
• Assay: Sandwich ELISA
• Conjugate: HRP
• Product Type: Antibody Pairs
• Specificity: This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human PD-L1.
• Target Species: Human
• For Use With (Application): Sandwich ELISA
• Gene Alias: B7-H, B7-H1, B7H1PDCD1L1, CD274 antigenMGC142294, CD274 molecule, PDCD1LG1MGC142296, PD-L1, PD-L1B7 homolog 1, PDL1PDCD1 ligand 1, programmed cell death 1 ligand 1, Programmed death ligand 1
• Gene ID (Entrez): 29126
• Gene Symbol: CD274
• Host Species: Mouse
• Kit Contents:
  Detection Antibody: 0.2 mg/mL of mouse anti-Human PD-L1 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4C).
  Capture Antibody: 1 mg/mL of mouse anti-Human PD-L1 monoclonal antibody (in PBS, pH 7.4).
  Standard: Each vial contains 65 ng of recombinant Human PD-L1.
• Quantity: 15 Plates
• Regulatory Status: RUO
• Research Discipline: Signal Transduction
• Storage Requirements: Storage is content dependent.
• Target: PD-L1
• Technique: 1. Capture Antibody: Dilute to a working concentration of 2 μg/mL in PBS before coating. 2. Detection Antibody: Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. 3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20 to -80°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 400 pg/mL is recommended.