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Invitrogen™ PD-L1 (CD274) Recombinant Rabbit Monoclonal Antibody (RM320), Alexa Fluor™ Plus 647

Catalog No. 756880394
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75-688-0394 500 μL
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Catalog No. 75-688-0394 Supplier Invitrogen™ Supplier No. 756880394
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Description

Rabbit Recombinant Monoclonal Antibody

Description: The RM320 monoclonal antibody reacts with human B7-H1, also known as PD-L1. B7-H1, a member of the B7 family, has a predicted molecular weight of approximately 40 kDa and belongs to the Ig superfamily. B7-H1 is expressed on a majority of leukocytes. B7-H1 is a ligand for PD-1. Interaction of PD-1 with either PD-L1 (B7-H1) or PD-L2 (B7-DC) results in inhibition of T and B cell responses. MIH1 is reported to be a blocking antibody. Applications Reported: This RM320 antibody has been tested by immunoblotting, immunohistochemistry, immunocytochemistry and flow cytometry. Applications Tested: This RM320 antibody has been tested by immunohistochemical staining of formalin-fixed paraffin embedded human tissue using low pH antigen retrieval and can be used at 5 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.

Programmed death receptor ligand 1 (PD-L1, also called B7-H1) is a recently described B7 family member. To date, one specific receptor has been identified that can be ligated by PD-L1. This receptor, programmed death receptor 1 (PD-1), has been shown to negatively regulate T-cell receptor (TCR) signaling. Upon ligating its receptor, PD-L1 has been reported to decrease TCR-mediated proliferation and cytokine production. PD-L1 expression was found to be abundant on many murine and human cancers and could be further up-regulated upon IFN-gamma stimulation. Thus, PD-L1 might play an important role in tumor immune evasion.
TRUSTED_SUSTAINABILITY

Specifications

Antigen PD-L1 (CD274)
Applications Immunohistochemistry (Paraffin), Multiplex Immunohistochemistry
Classification Recombinant Monoclonal
Clone RM320
Concentration 0.2 mg/mL
Conjugate Alexa Fluor Plus 647
Formulation PBS with 0.5% BSA, 10% proprietary stabilizer and 0.05% sodium azide; pH 7.2
Gene Cd274
Gene Accession No. Q9NZQ7
Gene Alias A530045L16Rik; B7 homolog 1; B7-H; B7h1; B7-H1; Cd274; CD274 antigen; CD274 molecule; PDCD1 ligand 1; Pdcd1l1; Pdcd1lg1; Pdl1; PD-L1; pdl-1; Programmed cell death 1 ligand 1; programmed death ligand 1; RGD1566211; sCD274; soluble CD274; soluble PD L1; sPD L1
Gene Symbols Cd274
Host Species Rabbit
Immunogen A peptide corresponding to the C-terminus of human PD-L1.
Purification Method Affinity chromatography
Quantity 500 μL
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 29126
Target Species Human
Content And Storage 4°C, store in dark
Product Type Antibody
Form Liquid
Isotype IgG
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