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Invitrogen™ PD-L1 Polyclonal Antibody
Rabbit Polyclonal Antibody
Supplier: Invitrogen™ PA520343
Description
This antibody detects human PD-L1 isoform 1 (290aa, 33.3 kDa) and isoform 3 (178aa, 20.5 kDa), as well as mouse and rat PD-L1 (290aa, 33 kDa). It will not cross-react with PD-L2. By Western blot, this antibody has been observed to detect a band at 37 kDa. Recommended positive controls: HeLa cell lysate, Daudi cell lysate, K562 cell lysate, human heart tissue/lysate. PA5-20343 can be used with blocking peptide PEP-0463. The PA5-20343 immunogen is located within amino acids 60-110 of PD-L1.
Programmed death receptor ligand 1 (PD-L1, also called B7-H1) is a recently described B7 family member. To date, one specific receptor has been identified that can be ligated by PD-L1. This receptor, programmed death receptor 1 (PD-1), has been shown to negatively regulate T-cell receptor (TCR) signaling. Upon ligating its receptor, PD-L1 has been reported to decrease TCR-mediated proliferation and cytokine production. PD-L1 expression was found to be abundant on many murine and human cancers and could be further up-regulated upon IFN-gamma stimulation. Thus, PD-L1 might play an important role in tumor immune evasion.
Specifications
PD-L1 | |
Polyclonal | |
Unconjugated | |
Cd274 | |
A530045L16Rik; B7 homolog 1; B7-H; B7h1; B7-H1; Cd274; CD274 antigen; CD274 molecule; PDCD1 ligand 1; Pdcd1l1; Pdcd1lg1; Pdl1; PD-L1; pdl-1; Programmed cell death 1 ligand 1; programmed death ligand 1; RGD1566211; sCD274; soluble CD274; soluble PD L1; sPD L1 | |
Rabbit | |
Antigen affinity chromatography | |
RUO | |
29126, 499342, 60533 | |
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. | |
Liquid |
Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry | |
1 mg/mL | |
PBS with 0.05% sodium azide | |
Q9EP73, Q9NZQ7 | |
Cd274 | |
A 17 amino acid peptide from near the center of human PDL-1. | |
100 μg | |
Primary | |
Human, Mouse, Rat | |
Antibody | |
IgG |
Safety and Handling
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