Invitrogen™ Human PDGF-AB ELISA Kit

Description

ELISA

The Human Platelet Derived Growth Factor-AB (Hu PDGFAB) ELISA quantitates Hu PDGFAB in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu PDGFAB. Principle of the method The Human PDGFAB solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Platelet-Derived Growth Factor (PDGF) is a mitogenic peptide growth hormone carried in the alpha-granules of platelets and is released when platelets adhere to traumatized tissues. Connective tissue cells near the traumatized region respond by initiating the process of replication. The synthesis of PDGF can be induced by IL-1, IL-6, TNF-alpha, TGF-beta and EGF.

Specifications

• Accession Number: P01127,P04085
• Assay Range: 10.24 to 2500 pg/mL
• Assay Sensitivity: 10 pg/mL
• Calibration Range: 10 pg/mL to 2500 pg/mL
• Assay: Solid-phase sandwich ELISA
• Conjugate: HRP
• Product Type: ELISA
• Protein Family: Growth Factors & Receptors
• Sample Type: Plasma, Serum, Supernatant
• Specificity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF-BB, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF- alpha, TNF-beta, TPO, VEGF
• Sufficient For: 10 x 96 assays
• Target Species: Human
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 5154, 5155
• Gene Symbol: IBGC5,PDGF-2,PDGF2,SIS,SSV,c-sis,PDGF-A,PDGF1
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 10 x 96 Tests
• Regulatory Status: RUO
• Research Discipline: Neurobiology
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
• Storage Requirements: 2°C to 8°C
• Synonym: LL22NC03-10C3.2, IBGC5, PDGF-2, PDGF2, SIS, SSV, c-sis
• Target: PDGF-AB
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins