Invitrogen™ PDHA1 Monoclonal Antibody (9H9AF5)

Catalog No.	Quantity
45-940-0	100 μg

Catalog No. 45-940-0 Supplier Invitrogen™ Supplier No. 459400

Description

Mouse Monoclonal Antibody

For positive control, use isolated mitochondria from Human, Bovine, Rat and Mouse hearts, HepG2 lysate; cultured, normal Human embryonic lung fibroblasts (strain MRC5); HL60 cells.

The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Specifications

• Antigen: PDHA1
• Applications: Flow Cytometry, Western Blot, Immunocytochemistry
• Classification: Monoclonal
• Clone: 9H9AF5
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: HEPES buffered saline with 0.02% sodium azide
• Gene: PDHA1
• Gene Accession No.: A7MB35, P08559, P26284, P35486
• Gene Alias: PDHA; PDHA1; Pdha-1; PDHAD; PDHCE1A; PDHE1-A type I; PHE1A; pyruvate dehydrogenase; pyruvate dehydrogenase (lipoamide) alpha 1; pyruvate dehydrogenase alpha 1; pyruvate dehydrogenase complex, E1-alpha polypeptide 1; pyruvate dehydrogenase E1 alpha 1; pyruvate dehydrogenase E1 alpha 1 subunit; pyruvate dehydrogenase E1 component alpha subunit; pyruvate dehydrogenase E1 component alpha subunit precursor; pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial; pyruvate dehydrogenase E1 subunit; pyruvate dehydrogenase E1 subunit alpha 1; pyruvate dehydrogenase E1alpha subunit
• Gene Symbols: PDHA1
• Host Species: Mouse
• Immunogen: Human recombinant full-length PDH E1-alpha.
• Purification Method: IgG fraction
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 18597, 29554, 407109, 5160
• Target Species: Human, Mouse, Rat, Bovine
• Content And Storage: 4°C, do not freeze
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1 κ