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Peroxidase Polyclonal Antibody, Rockland™
SDP

Catalog No. 501991014
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100 μg
25 μL
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Catalog No. Quantity
50-199-1014 25 μL
50-199-1013 100 μg
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Catalog No. 50-199-1014 Supplier Rockland Immunochemicals Supplier No. 2004138S
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Description

Rabbit Polyclonal Antibody

Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Anti-PEROXIDASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Peroxidase [Horseradish]. Cross reactivity against Peroxidase from other tissues and species may occur but have not been specifically determined.

Chemiluminescent detection systems have emerged as the best all-around method for detection of Western blots. They eliminate the hazards associated with radioactive materials and toxic chromogenic substrates. The speed and sensitivity of these methods are unequalled by traditional alternatives. Because results are generated on film, it is possible to record and store data permanently, and blots detected with chemiluminescent methods are easily stripped for subsequent reprobing with additional antibodies. HRP (Horseradish Peroxidase) conjugated secondary antibodies are utilized in conjunction with specific chemiluminescent substrates to generate the light signal. HRP-antibody conjugates have a very high turnover rate, giving good sensitivity with short reaction times.

Specifications

Antigen Peroxidase
Applications ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Classification Polyclonal
Concentration 1 mg/mL
Conjugate Unconjugated
Formulation 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Gene Alias Horseradish Peroxidase
Host Species Rabbit
Immunogen Peroxidase [Horseradish]
Quantity 25 μL
Regulatory Status RUO
Primary or Secondary Primary
Content And Storage Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Product Type Antibody
Form Lyophilized
Target Molecule Tag
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