Invitrogen™ Human PGI ELISA Kit

Description

ELISA

Pepsinogen is the inactive precursor form of Pepsin, one of the main proteolytic enzymes secreted by the gastric mucosa. Pepsinogen is synthesized in the gastric mucosa by chief cells. After secretion, pepsinogen is activated in the presence of HCl, which creates an acidic environment in the stomach, allowing pepsinogen to unfold and cleave itself in an autocatalytic fashion. Pepsin consists of a single polypeptide chain and arises from its precursor, pepsinogen, by removal of a 41 amino acid segment from the N-terminus.

Specifications

• Accession Number: P0DJD7
• Assay Range: 24.58 to 6000 pg/mL
• Assay Sensitivity: 20 pg/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 643847
• Gene Symbol: PGA4
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 10 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 100 μL, Serum 100 μL, Supernatant 100 μL
• Storage Requirements: 2°C to 8°C
• Target: PGI
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins