Invitrogen™ PGP9.5 Polyclonal Antibody

Catalog No.	Quantity
PIPA529012	100 μL

Catalog No. PIPA529012 Supplier Invitrogen™ Supplier No. PA529012

Description

Rabbit Polyclonal Antibody

Recommended positive controls: 293T, A549, H1299, Neuro2A, GL261, rat brain, PC-12, mouse brain. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

PGP9.5 (Protein gene product 9.5, UCH-L1, PARK5) is a neuron specific protein, structurally and immunologically distinct from neuron specific enolase. PGP9.5 has a molecular weight of 27 kDa and was first identified by high resolution two dimensional PAGE. PGP9.5 is a member of ubiquitin carboxyl-terminal hydrolase family 1 (peptidase family C12) with a ubiquitin carboxyl-terminal hydrolase domain. PGP9.5 is well known for having ubiquitin hydrolase and ligase activities that hydrolyzes small C-terminal adducts of ubiquitin to generate ubiquitin monomers. PGP9.5 is present in neurons and nerve fibers at all levels of the central and peripheral nervous system, in neuroendocrine cells, in segments of the renal tubules, in spermatogonia and Leydig cells of the testis, in ova and in some cells of both the pregnant and non-pregnant corpus luteum. Over expression of PGP9.5 leads to non-small cell lung cancer while decreased expression leads to Huntington disease and Alzheimer disease. Since PGP9.5 is present in cellular inclusions, it can be a useful as a neuronal marker and in the studies of neurodegenerative disorders such as with Parkinson disease.

Specifications

• Antigen: PGP9.5
• Applications: Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Classification: Polyclonal
• Concentration: 0.29 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 1% BSA, 20% glycerol and 0.025% ProClin 300; pH 7
• Gene: Uchl1
• Gene Accession No.: P09936, Q00981, Q9R0P9
• Gene Alias: AW822034; C88048; epididymis luminal protein 117; epididymis secretory protein Li 53; gad; gracile axonal dystrophy; HEL-117; HEL-S-53; MGC133697 protein; NDGOA; neuron cytoplasmic protein 9.5; OTTHUMP00000218137; OTTHUMP00000218139; OTTHUMP00000218140; OTTHUMP00000218141; PARK5; PGP 9.5; PGP9.5; PGP95; protein gene product 9.5; R75593; ubiquitin carboxyl-terminal esterase; ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase); ubiquitin carboxyl-terminal hydrolase isozyme L1; ubiquitin carboxyl-terminal hydrolase L1; ubiquitin carboxy-terminal hydrolase L1; ubiquitin C-terminal hydrolase; ubiquitin C-terminal hydrolase 1; ubiquitin C-terminal hydrolase L1; ubiquitin thioesterase L1; ubiquitin thiolesterase; ubiquitin thiolesterase L1; UCHL; UCHL1; UCH-L1; UCHL-1
• Gene Symbols: Uchl1
• Host Species: Rabbit
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PGP9.5. The exact sequence is proprietary.
• Purification Method: Antigen affinity chromatography
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 22223, 29545, 7345
• Target Species: Human, Mouse, Rat
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG

Frequently Asked Questions (FAQs)

Do you offer an alternative for PGP9.5 Polyclonal Antibody (Cat. No. PA5-16825)?

A suggested replacement for PGP9.5 Polyclonal Antibody (Cat. No. PA5-16825), 500 µL, is PGP9.5 Polyclonal Antibody (Cat. No. PA5-29012), 100 µL. The main difference between Cat. No. PA5-16825 and Cat. No. PA5-29012, is that Cat. No. PA5-16825 immunogen is a recombinant full-length human PGP9.5 protein, while the alternative Cat. No. PA5-29012 immunogen is a synthetic peptide corresponding to a region within amino acids 137 and 223 of the human PGP9.5 protein.

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov