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Cell Biolabs Inc CytoSelect™ 96-Well Phagocytosis Assay Kit (Red Blood Cell, Colorimetric), 96 assays
Supplier: Cell Biolabs Inc CBA220
Erythrocytes (RBCs) are commonly used in phagocytosis assays. For FcR mediated phagocytosis, RBCs are opsonized with serum or IgG before they are added to phagocytes. After removal of non-phagocytic erythrocytes, engulfed RBCs per phagocyte cell are manually counted (expressed as phagocytosis index or engulfed RBCs per phagocyte). This manual counting method is difficult when testing a large number of samples. CytoSelect™ 96-well Phagocytosis Assay does not involve subjective manual counting of RBCs. Instead cells are lysed and detected by the proprietary erythrocyte substrate in a microtiter plate reader. This format provides a quantitative, high-throughput method to accurately measure phagocytosis. The CytoSelect™ 96-well Phagocytosis Assay provides a robust system for screening TLR ligands, phagocytosis activators or inhibitors. Each kit provides sufficient quantities to perform 96, 48, 24 tests in a 96, 48, or 24-well plate, respectively. RBCs not included.
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