Invitrogen™ PHD1 Recombinant Rabbit Monoclonal Antibody (SP00-48)

Rabbit Recombinant Monoclonal Antibody

Supplier:  Invitrogen™ MA532043

Catalog No. PIMA532043

Description

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Prolyl hydroxylase domain proteins HIF PHD1, HIF PHD2 and HIF PHD3 (known as PHD1, PHD2 and PHD3 in rodents, respectively) can hydroxylate HIF-alpha subunits. Hypoxia-inducible factor (HIF) is a transcriptional regulator important in several aspects of oxygen homeostasis. The prolyl hydroxylases catalyze the posttranslational formation of 4-hydroxyproline in HIF-alpha proteins. HIF PHD1, which is widely expressed, with highest levels of expression in testis, functions as a cellular oxygen sensor and is important in cell growth regulation. HIF PHD1 can localize to the nucleus or the cytoplasm and is also detected in hormone responsive tissues, such as normal and cancerous mammary, ovarian and prostate epithelium. HIF PHD1 is encoded by EGLN2, which maps to chromosome 19q13. 3.

Specifications

• Antigen: PHD1
• Classification: Recombinant Monoclonal
• Concentration: 1 mg/mL
• Formulation: TBS with 0.05% BSA, 40% Glycerol and 0.05% sodium azide; pH 7.4
• Gene Accession No.: Q6AYU4, Q91YE2, Q96KS0
• Gene Symbols: EGLN2
• Immunogen: Recombinant protein within Human PHD1 aa 1-91.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG

• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry, Western Blot
• Clone: SP00-48
• Conjugate: Unconjugated
• Gene: EGLN2
• Gene Alias: 0610011A13Rik; C85656; egl nine homolog 2; egl-9 family hypoxia inducible factor 2; egl-9 family hypoxia-inducible factor 2; Egln2; EIT6; EIT-6; Estrogen-induced tag 6; falkor; HIF-1alpha prolyl-4-hydroxylase-1; HIF-P4H 1; Hif-p4h-1; HIFPH1; HIF-PH1; HIF-prolyl hydroxylase 1; HPH-1; HPH-3; Hypoxia-inducible factor prolyl hydroxylase 1; Ier4; immediate early response 4; P4H1; Phd1; PHD-1; Prolyl hydroxylase domain-containing protein 1; SM-20
• Host Species: Rabbit
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 112398, 112406, 308457
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid