Thermo Scientific Chemicals Phenol:Chloroform:Isoamyl alcohol 25:24:1, Ready-to-Use saturated aq. soln., pH 5.2

Description

Phenol-Chloroform-Isoamyl alcohol mixture in the ratio of 25:24:1 (v/v/v), CAS # 136112-00-0, 67-66-3, 123-51-3, is a solution used in the purification of nucleic acids.

Phenol:Chloroform:Isoamyl alcohol 25:24:1 is used in the extraction of DNA for the generation of DNA minisatellite fingerprints. It is also used for purifying RNA.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

General Description

• This solution contains a mixture of pure chloroform, isoamyl alcohol and phenol saturated with Tris-EDTA, with a stabilized pH of 8.0

Applications

• Phenol-Chloroform-Isoamyl alcohol mixture solution (25:24:1) is used in the extraction of nucleic acids, DNA and RNA
• When nucleic acids are extracted with the Phenol-Chloroform-Isoamyl alcohol mixture solution, a clear component separation is achieved. The proteins present in the sample are denatured and removed (collected in the organic phase or in the interphase), while nucleic acids remain in the aqueous phase. The chloroform denatures proteins and facilitates the separation of the aqueous and organic phases, and the isoamyl alcohol reduces foaming during extraction.
• It is also used to generate DNA minisatellite fingerprints

Specifications

Chemical Identifiers

• CAS: 136112-00-0
• Molecular Formula: C12H19Cl3O2
• Molecular Weight (g/mol): 301.632
• MDL Number: MFCD00133763
• InChI Key: ZYWFEOZQIUMEGL-UHFFFAOYSA-N
• Synonym: phenol-chloroform-isoamyl alcohol mixture, chloroform; isoamyl alcohol; phenol, phenol chloroform isoamyl alcohol, phenol-chloroform-isoamyl alcohol mixture, bioultra, for molecular biology, 125:24:1, phenol-chloroform-isoamyl alcohol mixture, bioultra, for molecular biology, 25:24:1, phenol-chloroform-isoamyl alcohol mixture, vetec tm reagent grade, 25:24:1, phenol-chloroform-isoamyl alcohol mixture, bioultra, for molecular biology, 49.5:49.5:1, phenol:chloroform:isoamyl alcohol 25:24:1, ready-to-use saturated aqueous solution, ph 5.2, with alkaline buffer, phenol:chloroform:isoamyl alcohol 25:24:1, ready-to-use saturated aqueous solution, ph 6.7, with alkaline buffer
• PubChem CID: 66587205
• IUPAC Name: chloroform;3-methylbutan-1-ol;phenol
• SMILES: CC(C)CCO.C1=CC=C(C=C1)O.C(Cl)(Cl)Cl

• Color: Colorless
• UN Number: UN2922
• Solubility Information: Soluble in water.
• pH: 5.2
• Quantity: 400 mL
• Physical Form: Liquid
• Chemical Name or Material: Phenol:Chloroform:Isoamyl alcohol 25:24:1, Ready-to-Use saturated aq. soln.

Safety and Handling

Hazard Category	Danger
Hazard Statement	GHS H Statement H331-H314-H341-H351-H373-H302-H312 Toxic if inhaled. Causes severe skin burns and eye damage. Suspected of causing genetic defects. Suspected of causing cancer. May cause damage to organs through prolonged or repeated exposure. Harmful if swallowed. Harmful in contact with skin.
Precautionary Statement	GHS P Statement P260-P303+P361+P353-P305+P351+P338-P301+P330+P331-P405-P501a Do not breathe dust/fume/gas/mist/vapors/spray. IF ON SKIN (or hair): Remove/Take off immediately all contaminated clothing. Rinse skin with water/shower. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. IF SWALLOWED: Rinse mouth. Do NOT induce vomiting. Store locked up. Dispose of contents/container in accordance with local/regional/national/international regulations.
DOTInformation	Transport Hazard Class: 8; Packing Group: II; Proper Shipping Name: CORROSIVE LIQUIDS, TOXIC, N.O.S.
TSCA	No
Recommended Storage	Keep cold

SDS

RUO – Research Use Only