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Invitrogen™ Phosphate Sensor

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Quantity:
10 nmol
100 nmol; 10,000 assay(s)
Description
The Phosphate Sensor is a highly sensitive reagent for the detection of inorganic phosphate, either in real-time kinetic or end-point mode. This product is a purified form of recombinant E. coli phosphate-binding protein labeled with the fluorophore MDCC, which is sensitive to changes in its environment. Binding of inorganic phosphate by Phosphate Sensor is rapid and tight (Kd ∼ 0.1μM), resulting in a large increase in fluorescence. The Phosphate Sensor detects phosphate in the high-nanomolar to low-micromolar range, which is 100-fold more sensitive than malachite green. This reagent provides you with a sensitive assay for detecting enzymes that produce inorganic phosphate either directly or indirectly through coupled reactions, such as phosphatases, ATPases, GTPases, and phosphodiesterases.

Specifications
Specifications
For Use With (Equipment) | Microplate Reader |
Target | GTPases, ATPases, Phosphatases, Phosphodiesterases |
For Use With (Application) | Fluorescence Intensity |
Assay | Phosphatase Assay |
Content And Storage | Phosphate Sensor is supplied as a 500 μM solution in 10 mM Tris (pH 7.6) and 50 mM NaCl. Store product at -80°C. |
Detection Method | Fluorescence |
Label or Dye | MDCC |
Product Type | Phosphate Sensor |
Quantity | 100 nmol; 10,000 assay(s) |
Format | 384-well plate |
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Safety and Handling
WARNING: Cancer - www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.
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