Invitrogen™ Phosphate Sensor

Phosphate Sensor

Supplier:  Invitrogen™ PV4407

Catalog No. PV4407

Description

The Phosphate Sensor is a highly sensitive reagent for the detection of inorganic phosphate, either in real-time kinetic or end-point mode. This product is a purified form of recombinant E. coli phosphate-binding protein labeled with the fluorophore MDCC, which is sensitive to changes in its environment. Binding of inorganic phosphate by Phosphate Sensor is rapid and tight (Kd ∼ 0.1μM), resulting in a large increase in fluorescence. The Phosphate Sensor detects phosphate in the high-nanomolar to low-micromolar range, which is 100-fold more sensitive than malachite green. This reagent provides you with a sensitive assay for detecting enzymes that produce inorganic phosphate either directly or indirectly through coupled reactions, such as phosphatases, ATPases, GTPases, and phosphodiesterases.

Specifications

• For Use With (Equipment): Microplate Reader
• For Use With (Application): Fluorescence Intensity
• Content And Storage: Phosphate Sensor is supplied as a 500 µM solution in 10 mM Tris (pH 7.6) and 50 mM NaCl. Store product at -80°C.
• Label or Dye: MDCC
• Quantity: 100 nmol
• Excitation/Emission: 430⁄450
• Shipping Condition: Dry Ice

• Target: GTPases, ATPases, Phosphatases, Phosphodiesterases
• Assay: Phosphatase Assay
• Detection Method: Fluorescent
• Product Type: Phosphate Sensor
• Format: 384-well plate
• Readout: Kinetic

For Research Use Only. Not for use in diagnostic procedures.