Invitrogen™ Phosphate Sensor

Phosphate Sensor

Manufacturer:  Invitrogen™ PV4407

Catalog No. PV4407

Description

The Phosphate Sensor is a highly sensitive reagent for the detection of inorganic phosphate, either in real-time kinetic or end-point mode. This product is a purified form of recombinant E. coli phosphate-binding protein labeled with the fluorophore MDCC, which is sensitive to changes in its environment. Binding of inorganic phosphate by Phosphate Sensor is rapid and tight (Kd ∼ 0.1μM), resulting in a large increase in fluorescence. The Phosphate Sensor detects phosphate in the high-nanomolar to low-micromolar range, which is 100-fold more sensitive than malachite green. This reagent provides you with a sensitive assay for detecting enzymes that produce inorganic phosphate either directly or indirectly through coupled reactions, such as phosphatases, ATPases, GTPases, and phosphodiesterases.

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Specifications

• Assay: Phosphatase Assay
• For Use With (Equipment): Microplate Reader
• Target Enzyme: GTPases, ATPases, Phosphatases, Phosphodiesterases
• Product Type: Phosphate Sensor
• Excitation/Emission: 430⁄450
• Shipping Condition: Dry Ice
• Label or Dye: MDCC

• Form: Liquid
• Format: 384-well plate
• Technique: Fluorescence Intensity
• Quantity: 100nmol
• Readout: Kinetic
• Detection Method: Fluorescent

For Research Use Only. Not for use in diagnostic procedures.