Invitrogen™ Phospho-AIF (Ser116) Polyclonal Antibody

Catalog No.	Quantity
PIPA5143645	100 μL

Catalog No. PIPA5143645 Supplier Invitrogen™ Supplier No. PA5143645

Description

Rabbit Polyclonal Antibody

The antibody was cross adsorbed to unphosphorylated AIFM1 (Ser-116) peptide before affinity purification using phospho-AIFM1 (Ser-116) peptide. This antibody antibody detects a triplet at 66, 62, and 57 kDa* protein on SDS-PAGE immunoblots of human Jurkat cells treated with calyculin A. This reactivity is not observed after lambda phosphatase treatment to dephosphorylated AIFM1.

Apoptosis Inducing Factor (AIF) causes chromatin condensation and DNA fragmentation. AIF was recently identified, and cloned. Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins. Like the critical molecules, cytochrome c and caspase-9, in apoptosis, AIF localizes in mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. AIF induces chromatin condensation and large scale DNA fragmentation, which are the hallmarks of apoptosis, of the isolated nucleus and the nucleus in live cells by microinjection and apoptosis stimuli. AIF is highly conserved between human and mouse and widely expressed. Mutations in the AIF gene cause combined oxidative phosphorylation deficiency 6, which results in a severe mitochondrial encephalomyopathy. Alternative splicing results in multiple transcript variants of AIF and a related pseudogene has been identified on chromosome 10.

Specifications

• Antigen: Phospho-AIF (Ser116)
• Applications: Western Blot
• Classification: Polyclonal
• Concentration: 0.5 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 1 mg/mL BSA, 50% glycerol and 0.05% sodium azide
• Gene: AIFM1
• Gene Accession No.: O95831
• Gene Alias: AG1; AGR1; Aif; AIFM1; AIFsh2; apoptosis inducing factor mitochondria associated 1; apoptosis inducing factor, mitochondria associated 1; apoptosis-inducing factor 1, mitochondrial; apoptosis-inducing factor, mitochondrion-associated 1; apoptosis-inducing factor, mitochondrion-associated, 1; CMT2D; CMTX4; COWCK; COXPD6; DFNX5; ER protein 18; ER protein 19; ERP18; ERP19; hAG-1; harlequin; Hq; hTLP19; MGC111425; mitochondrial apoptosis-inducing factor 1; NADMR; NAMSD; pcd8; PDCD8; PDIA16; programmed cell death 8 (apoptosis-inducing factor); programmed cell death protein 8; RP3-438D16.2; striatal apoptosis-inducing factor; testicular secretory protein Li 4; TLP19; UNQ713/PRO1376
• Gene Symbols: AIFM1
• Host Species: Rabbit
• Immunogen: Phospho-AIFM1 (Ser-116) synthetic peptide (coupled to carrier) corresponding to amino acids surrounding Ser-116 in human AIFM1. This site is conserved in rat and mouse AIFM1, but the amino acids surrounding the site are not well conserved. The site is not found in AIFM2 and AIFM3.
• Purification Method: Antigen affinity chromatography
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 9131
• Target Species: Human
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG