Invitrogen™ Phospho-alpha Synuclein (Ser129) Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA577815

Catalog No. PIPA577815

Description

A 1:1,000 dilution of PA5-77815 was sufficient for detection of Alpha Synuclein pSer129 in 15 μg of human brain cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody. Binds to phosphorylated serine 129 on alpha synuclein. Does not detect unphosphorylated serine 129 alpha synuclein. Detects bands at 100, 75, 45, 15 kDa. Bands above 15 kDa are oligomers.

Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. These proteins are abundant in the brain and play a role in regulating synaptic transmission and membrane trafficking. Alpha- and beta-synuclein have been shown to selectively inhibit phospholipase D2, suggesting that they may also be involved in signaling pathways. Defects in the SNCA gene, which encodes alpha-synuclein, have been implicated in the pathogenesis of Parkinson's disease. Additionally, alpha-synuclein peptides are a major component of amyloid plaques in the brains of patients with Alzheimer's disease. Multiple alternatively spliced transcripts encoding different isoforms have been identified for this gene. Diseases associated with SNCA include Parkinson Disease 1, Autosomal Dominant and Dementia, Lewy Body.

Specifications

• Antigen: Phospho-alpha Synuclein (Ser129)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: SNCA
• Gene Alias: Alpha synuclein; alphaSYN; alpha-synuclein; NACP; non A-beta component of AD amyloid; non-A beta component of AD amyloid; non-A4 component of amyloid; Non-A4 component of amyloid precursor; PARK1; PARK4; PD1; Snca; Syn; synuclein alpha; synuclein alpha-140; synuclein, alpha; synuclein, alpha (non A4 component of amyloid precursor)
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 20617, 29219, 6622
• Content And Storage: -20°C
• Form: Liquid

• Applications: ELISA, Immunohistochemistry (Paraffin), Immunohistochemistry (PFA fixed), Western Blot, Immunocytochemistry
• Concentration: 0.5 mg/mL
• Formulation: PBS with 50% glycerol and 0.09% sodium azide; pH 7.4
• Gene Accession No.: O55042, P37377, P37840
• Gene Symbols: SNCA
• Immunogen: Synthetic peptide of Human Alpha Synuclein pSer129 (40-140 aa), conjugated to Keyhole Limpet Haemocyanin (KLH).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

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