Invitrogen™ Phospho-ATF2 (Ser490, Ser498) Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA14613

Catalog No. PA14613

Description

This antibody is predicted to react with rat based on 100% sequence homology. This antibody is specific for ~74 kDa ATF2 protein phosphorylated at Ser490,498. It also recognizes the phosphorylated ~54 kDa splice form of ATF2. Store at -20°C short term, 80°C long term.

The transcription factor ATF-2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins. ATF-2 interacts with a variety of viral oncoproteins and cellular tumor suppressors and is a target of the SAPK/JNK and p38 MAP kinase signaling pathways. Various forms of cellular stress, including genotoxic agents, inflammatory cytokines and UV irradiation, stimulate the transcriptional activity of ATF-2. Cellular stress activates ATF-2 by phosphorylation of Thr69 and Thr71. Both SAPK and p38 MAPK have been shown to phosphorylate ATF-2 at these sites in vitro and in cells transfected with ATF-2.

Specifications

• Antigen: Phospho-ATF2 (Ser490, Ser498)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Atf2
• Gene Alias: Activating 2; Activating transcription factor 2; activating transcription factor 2 splice variant ATF2-var2; ATF2; Atf-2; cAMP response element-binding protein CRE-BP1; cAMP responsive element binding protein 2, formerly; cAMP-dependent transcription factor ATF-2; CREB2; CREB-2; CRE-BP; CREBP1; CRE-BP1; Cyclic AMP-dependent transcription factor ATF-2; cyclic AMP-responsive element-binding protein 2; D130078H02Rik; D18875; HB16; Histone acetyltransferase ATF2; MGC111558; mXBP; MXBP protein; OTTHUMP00000205345; OTTHUMP00000205347; OTTHUMP00000205348; OTTHUMP00000205350; OTTHUMP00000205352; OTTHUMP00000205353; OTTHUMP00000205354; Tg(Gzma-Klra1)7Wum; TREB7
• Host Species: Rabbit
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 11909, 1386
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: Immunohistochemistry, Western Blot, Immunocytochemistry
• Concentration: 0.25 mg/mL
• Formulation: 0.01M HEPES with 100 μg/mL BSA, 50% glycerol, 0.15M NaCl and no preservative; pH 7.5
• Gene Accession No.: P15336, P16951
• Gene Symbols: Atf2
• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser490/498 of human ATF2 conjugated to KLH.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG