Invitrogen™ Phospho-BCKDHA (Ser292) Recombinant Rabbit Monoclonal Antibody (BLR060F)

Rabbit Recombinant Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
PIMA544313	Invitrogen™ MA544313	100 μL
PIMA544314	Invitrogen™ MA544314	20 μL

Description

Phospho-BCKDHA (Ser292) Recombinant Monoclonal Antibody for Western Blot

The BCKDHA gene encodes the E1-alpha subunit of the branched-chain alpha-keto acid (BCAA) dehydrogenase complex (BCKD; EC 1.2.4.4), an inner-mitochondrial enzyme complex that catalyzes the oxidative decarboxylation of the branched-chain alpha-ketoacids derived from isoleucine, leucine, and valine. This reaction is the second major step in the catabolism of the branched-chain amino acids (Wynn et al., 1998). The BCKD complex consists of 3 catalytic components: a heterotetrameric (alpha2-beta2) branched-chain alpha-keto acid decarboxylase (E1), a homo-24-meric dihydrolipoyl transacylase (E2), and a homodimeric dihydrolipoamide dehydrogenase (E3). E1 is a thiamine pyrophosphate (TPP)-dependent enzyme. The reaction is irreversible and constitutes the first committed step in BCAA oxidation. The BCKDHB gene encodes the beta subunit of E1. The complex also contains 2 regulatory enzymes, a kinase and a phosphorylase.

Specifications

• Antigen: Phospho-BCKDHA (Ser292)
• Concentration: 40 μg/mL
• Applications: Western Blot
• Conjugate: Unconjugated
• Host Species: Rabbit
• Regulatory Status: RUO
• Formulation: BBS with 0.1% BSA and 0.09% sodium azide; pH 7.8-8.6
• Gene Accession No.: P12694, P50136
• Gene ID (Entrez): 12039, 593
• Immunogen: Peptide surrounding phosphorylated serine 292 of mitochondrial human Branched Chain Keto Acid Dehydrogenase E1, Alpha polypeptide.
• Product Type: Antibody

• Clone: BLR060F
• Content And Storage: 4°C
• Classification: Recombinant Monoclonal
• Form: Liquid
• Isotype: IgG
• Target Species: Human, Mouse
• Gene: BCKDHA
• Gene Alias: 2-oxoisovalerate dehydrogenase (lipoamide); 2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial; BCKAD E1[a]; BCKDA; BCKDE1A; BCKDH E1-alpha; BCKDHA; branched chain alpha-ketoacid dehydrogenase subunit E1 alpha; branched chain keto acid dehydrogenase E1 alpha protein; branched chain keto acid dehydrogenase E1, alpha polypeptide; branched chain keto acid dehydrogenase subunit E1, alpha polypeptide; branched chain ketoacid dehydrogenase E1, alpha polypeptide; branched-chain alpha-keto acid dehydrogenase E1 component alpha chain; E1a; MSU; MSUD1; OVD1A
• Gene Symbols: BCKDHA
• Primary or Secondary: Primary
• Purification Method: Purified