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Invitrogen™ Phospho-beta Catenin/gamma Catenin (Tyr489, Tyr480) Polyclonal Antibody
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Catalog No. PIPA5143666
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PIPA5143666 100 μL
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Catalog No. PIPA5143666 Supplier Invitrogen™ Supplier No. PA5143666
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Description

Rabbit Polyclonal Antibody

This antibody was cross-adsorbed to phospho-beta-Catenin (Tyr-654) peptide before affinity purification using phospho-beta-Catenin (Tyr-489) peptide (without carrier). The antibody detects 84 and 88kDa* proteins corresponding to the molecular mass of gamma-Catenin and beta-Catenin, respectively, on SDS-PAGE immunoblots of A431 cells treated with pervanadate, but does not detect these proteins in control cells.

beta-Catenin is a 92 kDa protein that binds to the cytoplasmic tail of E-Cadherin. The cadherins, transmembrane adhesion molecules, are found with catenins at adherens junctions. Deletions in the cytoplasmic domain of E-Cadherin eliminate catenin binding and result in a loss of cell adhesion. Tyrosine phosphorylation of beta-Catenin can regulate its interaction with critical components of adherens junctions. Both Fer and Fyn kinases phosphorylate tyrosine 142 in vitro. Overexpression of these kinases in epithelial cells disrupts interactions between alpha- and beta-Catenins. The phosphorylation of tyrosine 142 may act as a switch from the transcriptional to the adhesive role of beta-Catenin. Src family kinases can also phosphorylate tyrosine 86 and 654 in beta-Catenin. The Tyr-654 phosphorylation regulates beta-Catenin binding to E-cadherin. Thus, site-specific tyrosine phosphorylation of beta-Catenin may regulate protein-protein interactions leading to changes in cell adhesion.
TRUSTED_SUSTAINABILITY

Specifications

Antigen Phospho-beta Catenin/gamma Catenin (Tyr489, Tyr480)
Applications Western Blot, Immunocytochemistry
Classification Polyclonal
Concentration 0.5 mg/mL
Conjugate Unconjugated
Formulation PBS with 1mg/mL BSA, 50% glycerol and 0.05% sodium azide
Gene CTNNB1
Gene Accession No. P35222, Q02248, Q9WU82
Gene Alias Catenin beta1; CTNNB1
Gene Symbols CTNNB1
Host Species Rabbit
Immunogen Phospho-beta-Catenin (Tyr-489) synthetic peptide corresponds to amino acid residues around tyrosine 489 of human beta-Catenin. This peptide sequence is highly conserved in rat and mouse beta-Catenin, and has high homology to the conserved site in gamma-Catenin (Tyr-480).
Purification Method Antigen affinity chromatography
Quantity 100 μL
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 12387, 1499, 84353
Target Species Human, Mouse, Rat
Content And Storage -20°C, Avoid Freeze/Thaw Cycles
Product Type Antibody
Form Liquid
Isotype IgG
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