Invitrogen™ Phospho-c-Kit (Tyr730) Polyclonal Antibody

Catalog No.	Quantity
44-496-G	100 μL

Catalog No. 44-496-G Supplier Invitrogen™ Supplier No. 44496G

Description

Rabbit Polyclonal Antibody

Phospho-c-Kit (Tyr730) Polyclonal Antibody for IHC (P), Western Blot

KIT (c-KIT) is a proto-oncogene and a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). KIT was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. KIT together with its ligand regulates growth and activation of a variety of hemopoietic and non-hemopoietic cells. Mutations in KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. Recently, deregulation of the KIT receptor TK by the prevalent activation loop mutation D816V has served as a focal point in therapeutic strategies aimed at curbing neoplastic mast cell growth. c-Kit is expressed in hematopoietic stem cells, germ cells, mast cells and gastrointestinal tract cajal cells. Upon binding of its ligand stem cell factor (SCF), c-kit dimerizes, resulting in receptor activation and autophosphorylation of various tyrosine residues including tyrosine 703 located on the cytoplasmic domain of the receptor. This modification allows docking of Grb2 and activation of the Ras/ERK signaling pathway. SCF/c-kit can activate multiple downstream signaling pathways including PI3K, PLC-gamma and JAK/STAT. c-kit receptor activation is essential for hematopoiesis, stem cell maintenance and gametogenesis.

Specifications

• Antigen: Phospho-c-Kit (Tyr730)
• Applications: Immunohistochemistry (Paraffin), Western Blot
• Classification: Polyclonal
• Conjugate: Unconjugated
• Formulation: Dulbecco's PBS with 1mg/mL BSA and 0.05% sodium azide; pH 7.3
• Gene: KIT
• Gene Accession No.: O97799, P05532, P10721, P43481, Q08156
• Gene Alias: belly-spot; Bs; CD117; ckit; C-Kit; c-KIT gene1; c-kit protein; c-kit proto-oncogene protein; c-kit receptor; c-kit receptor tyrosine kinase; dominant spotting; Dominant white spotting; Fdc; Gsfsco1; Gsfsco5; Gsfsow3; KIT; kit oncogene; KIT proto-oncogene receptor tyrosine kinase; KIT proto-oncogene, receptor tyrosine kinase; mast cell growth factor receptor; mast/stem cell growth factor receptor; mast/stem cell growth factor receptor Kit; MGF; p145 c-kit; PBT; Piebald trait protein; protein kinase; proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; receptor tyrosine kinase c-kit; receptor tyrosine kinase type III; SCFR; SCO1; SCO5; Sl; soluble KIT variant 1; SOW3; spotted sterile male; Ssm; Steel Factor Receptor; Tr-kit; tyrosine kinase receptor; tyrosine kinase receptor c-KIT; tyrosine-protein kinase Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein; W
• Gene Symbols: KIT
• Host Species: Rabbit
• Immunogen: The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human c-Kit that contains tyrosine 730. The sequence is conserved in mouse, rat and cow.
• Purification Method: Antigen affinity chromatography
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 16590, 280832, 378783, 3815, 403811, 64030
• Target Species: Human, Mouse, Rat, Canine, Bovine, Chicken
• Content And Storage: -20°C
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG

Frequently Asked Questions (FAQs)

What happens when stem cell factor (SCF) binds to its receptor?

The interaction of SCF with its receptor SCFR (c-Kit) causes receptor dimerization followed by receptor phosphorylation on tyrosine residues in the cytoplasmic domain. This activates downstream signal transduction pathways involving PI-3 kinase/Akt, Src family kinase, Ras/MAP kinase, and Rac/JNK kinase. There are several tyrosine residues on c-Kit that are important in activation of downstream signal pathways:
[pTyr703]: Binds Grb2 and activates the Ras/MAPK pathway.
[pTyr730]: involved in PLC-gamma activation which leads to mitotic stimulation.
[pTyr936]: binds GRB2 and activates the Ras, Raf, and ERK1/2 signaling pathways.

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov