Invitrogen™ Phospho-Catenin alpha-1 (Tyr148) Polyclonal Antibody

Catalog No.	Quantity
PIPA5143662	100 μL

Catalog No. PIPA5143662 Supplier Invitrogen™ Supplier No. PA5143662

Description

Rabbit Polyclonal Antibody

This antibody was cross-adsorbed to unrelated phospho-tyrosine peptide before affinity purification using phospho-alpha1-Catenin (Tyr-148) peptide (without carrier). The antibody detects a 102 kDa* protein corresponding to the molecular mass of alpha1-Catenin on SDS-PAGE immunoblots of rat PC12 cells treated with pervanadate.

Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherence junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments. May play a crucial role in cell differentiation.

Specifications

• Antigen: Phospho-Catenin alpha-1 (Tyr148)
• Applications: Western Blot
• Classification: Polyclonal
• Concentration: 0.5 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 1mg/mL BSA, 50% glycerol and 0.05% sodium azide
• Gene: CTNNA1
• Gene Accession No.: P26231, P35221
• Gene Alias: [a]E-catenin; 102 kDa cadherin-associated protein; 2010010M04Rik; AA517462; AI988031; alpha E catenin; alpha E-catenin; alpha(E)-catenin; alpha-E-catenin; cadherin associated protein; Cadherin-associated protein; CAP102; catenin (cadherin associated protein), alpha 1; catenin (cadherin-associated protein), alpha; catenin (cadherin-associated protein), alpha 1; catenin (cadherin-associated protein), alpha 1, 102kDa; catenin alpha 1; catenin alpha 1 S homeolog; catenin alpha-1; catenin, alpha 1; Catna1; cell-adhesion protein alphaE catenin; ctnna; ctnna1; ctnna1.S; MDPT2; Renal carcinoma antigen NY-REN-13; XELAEV_18020018mg
• Gene Symbols: CTNNA1
• Host Species: Rabbit
• Immunogen: Phospho-alpha1-Catenin (Tyr-148) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 148 in human alpha1-Catenin. This peptide sequence is highly conserved in rat and mouse alpha1-Catenin, but is not conserved in alpha2-Catenin or alpha3-Catenin.
• Purification Method: Antigen affinity chromatography
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 12385, 1495, 307505
• Target Species: Human, Mouse, Rat
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG