Invitrogen™ Phospho-CD247 (CD3 zeta) (Tyr142) Monoclonal Antibody (EM-54)

Catalog No.	Quantity
PIMA528538	100 μg

Catalog No. PIMA528538 Supplier Invitrogen™ Supplier No. MA528538

Description

Mouse Monoclonal Antibody

This antibody recognizes phosphorylated intracellular tyrosine 142 of CD3 zeta chain (CD247), which is a component of TCR/CD3 complex expressed on T cells. Flow cytometry: Intracellular staining. Western blotting: non-reducing conditions.

CD3z complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules, and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. Diseases associated with CD3z include Immunodeficiency 25 and T-B+ Severe Combined Immunodeficiency Due To Cd3delta/Cd3epsilon/Cd3zeta.

Specifications

• Antigen: Phospho-CD247 (CD3 zeta) (Tyr142)
• Applications: Flow Cytometry, Western Blot
• Classification: Monoclonal
• Clone: EM-54
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 15mM sodium azide; pH 7.4
• Gene: CD247
• Gene Accession No.: P20963, P24161
• Gene Alias: 4930549J05Rik; A430104F18Rik; antigen CD3Z, zeta polypeptide; AW552088; Cd247; CD247 antigen; CD247 antigen, zeta subunit; Cd247 molecule; Cd3; CD3 antigen, zeta polypeptide; CD3 zeta; CD3 zeta chain; CD3e antigen; CD3-epsilon; Cd3-eta; CD3H; CD3Q; Cd3z; CD3Z antigen, zeta polypeptide (TiT3 complex); Cd3zeta; Cd3-zeta; CD3zeta chain; CD3-zeta/eta; FLJ18683; IMD25; T3E; T3z; T-cell antigen receptor complex, zeta subunit of CD3; T-cell receptor CD3 subunit zeta; T-cell receptor CD3, subunit zeta; T-cell receptor T3 eta chain; T-cell receptor T3 zeta chain; T-cell receptor zeta chain; T-cell surface glycoprotein CD3 zeta chain; TCR zeta chain; TCRE; Tcrk; TCRZ; TCRzeta
• Gene Symbols: CD247
• Host Species: Mouse
• Immunogen: CP1 (pTyr142) - KLH.
• Purification Method: Protein A
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 12503, 919
• Target Species: Human, Mouse
• Content And Storage: 4°C, do not freeze
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1