MilliporeSigma™ phospho Cofilin-1 (Ser287), Rabbit, Unlabeled, Polyclonal,

Catalog No.	Quantity	Conjugate
AB3831I	100 μL	Unconjugated

Catalog No. AB3831I Supplier MilliporeSigma™ Supplier No. AB3831I

Description

Rabbit Polyclonal Antibody

Cofilin-1 (UniProt P23528; also known as 18kDa phosphoprotein, Cofilin non-muscle isoform, p18) is encoded by the CFL1 (also known as CFL) gene (Gene ID 1072) in human. Cofilin-1 (non-muscle cofilin), cofilin-2 (muscle cofilin), and actin-depolymerizing factor (ADF) play an essential role in regulating actin filament dynamics and reorganization by stimulating the severance and depolymerization of actin filaments. The three cofilin family members share 70% to 81% sequence identity, exhibit similar biological functions, and display distinct but overlapping expression patterns. Cofilins and ADF bind actin monomers and filaments (G-actin and F-actin) and increase the number of actin monomers and filament fragments, facilitating filament turnover and treadmilling at key locations in migrating cells. Cofilins and ADF are inactivated by phosphorylation at Ser3 by LIM-kinases (LIMKs) and testicular protein kinases (TESKs) and are reactivated by dephosphorylation by the slingshot (SSH) family of protein phosphatases and chronophin. Cfl1-knockout results in mouse embryonic lethality at E11.5 12.5 due to aberrant neural tube closure and defective neural crest cell migration, while Adf-deficient mice are viable with normal brain appearance, but suffer from corneal defects that cause blindness in adult mice.

Specifications

• Antigen: phospho Cofilin-1 (Ser287)
• Applications: Western Blot
• Classification: Polyclonal
• Concentration: Please refer to lot specific datasheet.
• Conjugate: Unconjugated
• Formulation: Purified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
• Gene: CFL, CFL1
• Gene Accession No.: P23528
• Host Species: Rabbit
• Immunogen: KLH-conjugated linear peptide corresponding to the N-termnal sequence of human cofilin-1 with phosphorylated Ser3.
• Purification Method: Affinity Purified
• Quantity: 100 μL
• Regulatory Status: RUO
• Research Discipline: Cell Structure
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_005498
• Test Specificity: Target band detection by this polyclonal antibody was prevented by preblocking with the immunogen phosphopeptide, but not the corresponding non-phosphopeptide.
• Target Species: Human
• Content And Storage: Stable for 1 year at 2°-8°C from date of receipt.

For Research Use Only. Not for use in diagnostic procedures.