Phospho-E2F-1 (Ser364)Rabbit anti-Chimpanzee, Human, Polyclonal, Rockland™

Rabbit Polyclonal Antibody

Supplier:  Rockland Immunochemicals 600401690

Catalog No. 50-199-1796

Description

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. This affinity purified antibody is directed against the phosphorylated form of human E2F-1 at the pS364 residue. Reactivity occurs against human E2F-1 pS364 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human E2F-1 is minimal by ELISA. The antibody does not cross-react with E2F-1 phosphorylated at other sites. A BLAST analysis was used to suggest reactivity with this protein from human and chimpanzee based on 100% homology for the immunogen sequence. Cross reactivity with E2F-1 homologues from other sources has not been determined.

The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.

Specifications

• Antigen: Phospho-E2F-1 (Ser364)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: E2F1
• Gene Alias: E2F transcription factor 1; E2F1; E2F-1; mKIAA4009; PBR3; pRB-binding protein E2F-1; RBAP1; RBAP-1; RBBP3; RBBP-3; RBP3; Retinoblastoma-associated protein 1; Retinoblastoma-binding protein 3; Transcription factor E2F1
• Host Species: Rabbit
• Regulatory Status: RUO
• Gene ID (Entrez): 107969994, 1869, 458184
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ELISA, Immunohistochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
• Concentration: 1 mg/mL
• Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
• Gene Accession No.: Q01094
• Gene Symbols: E2F1
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Chimpanzee, Human
• Product Type: Antibody