Phospho Enol Pyruvate Carboxylase Polyclonal Antibody, Biotin, Rockland™

Rabbit Polyclonal Antibody

Supplier:  Rockland Immunochemicals 2004663S

Catalog No. 50-199-1189

Description

Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Anti-Phospho Enol Pyruvate Carboxylase antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Phospho-enol-Pyruvate-Carboxylase [Maize]. Cross reactivity against Phospho-enol-Pyruvate-Carboxylase from other sources may occur but have not been specifically determined.

Phosphoenolpyruvate Carboxylase, through the carboxylation of phosphoenolpyruvate (PEP), forms an oxaloacetate, a four-carbon dicarboxylic acid source for the tricarboxylic acid cycle. Phospho-enol-pyruvate Carboxylase enzyme is regulated by light-reversible phosphorylation. This protein is involved in the pathway C3 acid pathway, which is part of Photosynthesis.

Specifications

• Antigen: Phospho Enol Pyruvate Carboxylase
• Classification: Polyclonal
• Conjugate: Biotin
• Host Species: Rabbit
• Quantity: 25 μL
• Primary or Secondary: Primary
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Lyophilized

• Applications: ELISA, Immunoprecipitation, Western Blot
• Concentration: 1 mg/mL
• Formulation: 0.02M potassium phosphate with 0.15M NaCl, 10 mg/mL BSA and 0.01% sodium azide; pH 7.2
• Immunogen: Phospho-enol-pyruvate Carboxylase [Maize Leaves]
• Regulatory Status: RUO
• Target Species: Plant
• Product Type: Antibody