Invitrogen™ Phospho-eNOS (Ser632) Monoclonal Antibody (M232)

Catalog No.	Quantity
PIMA547632	100 μL

Catalog No. PIMA547632 Supplier Invitrogen™ Supplier No. MA547632

Description

Mouse Monoclonal Antibody

The antibody detects a 140 and 120 kDa* bands on SDS-PAGE immunoblots of human umbilical vein endothelial cells, but these bands are not observed after lambda phosphatase treatment. The 120 kDa band may be a truncated form of eNOS.

Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) and endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains.

Specifications

• Antigen: Phospho-eNOS (Ser632)
• Applications: Western Blot
• Classification: Monoclonal
• Clone: M232
• Concentration: 0.25 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 1mg/mL BSA, 50% glycerol and 0.05% sodium azide
• Gene: NOS3
• Gene Accession No.: P29474, P70313, Q62600
• Gene Alias: 2310065A03Rik; cNOS; Constitutive NOS; Ecnos; EC-NOS; endothelial nitric oxide synthase; endothelial nitric oxide synthase 3; endothelial nitric oxide synthase NOS3; endothelial NOS; eNos; e-NOS; nitric oxide synthase; nitric oxide synthase 3; nitric oxide synthase 3 (endothelial cell); nitric oxide synthase 3 transcript variant eNOS-delta20; nitric oxide synthase 3 transcript variant eNOS-delta20-21; nitric oxide synthase 3 transcript variant eNOS-delta21; nitric oxide synthase 3, endothelial cell; nitric oxide synthase, endothelial; NOS; NOS type III; Nos3; Nos-3; NOSIII; OTTHUMP00000213183
• Gene Symbols: NOS3
• Host Species: Mouse
• Immunogen: Clone M232 was generated from a Phospho-eNOS (Ser-632) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding Ser-632 in mouse eNOS. This sequence is conserved in human (Ser-633) and rat (Ser-632) eNOS, and has low homology to other NOS family members.
• Purification Method: Protein A
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 18127, 24600, 4846
• Target Species: Human, Mouse, Rat
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1