Invitrogen™ Phospho-FAK (Tyr576, Tyr577) Polyclonal Antibody

Catalog No.	Quantity
PIPA537706	100 μL

Catalog No. PIPA537706 Supplier Invitrogen™ Supplier No. PA537706

Description

Rabbit Polyclonal Antibody

A suggested positive control for Western blot is HT29 cells; suggested positive control for ICC/IF is Hela cells.

Focal Adhesion Kinase (FAK) is a 125 kDa non-receptor protein tyrosine kinase that acts as a substrate for Src and is a key element of integrin signaling. FAK plays an important role in cell spreading, differentiation, migration, cell death, and acceleration of the G1 to S phase transition of the cell cycle. FAK has a central catalytic domain and a C-terminal tail that localizes it to focal adhesions, which are sites where cells attach to the extracellular matrix via surface integrin receptors. Increased FAK tyrosine phosphorylation occurs upon integrin engagement with fibronectin. Adhesion of murine NIH3T3 fibroblasts to fibronectin promotes association of the Grb2 adapter protein and c-Src PTK with FAK in vivo, and also results in activation of the ERK2 MAP kinase. In v-Src-transformed NIH3T3, the association of v-Src, Grb2, and Sos with FAK is independent of cell adhesion to fibronectin. In vitro the Grb2 SH2 domain binds directly to tyrosine-phosphorylated FAK, and the binding site has been identified as Tyr925 by site directed mutagenesis. Several transcript variants encoding different isoforms have been found for the FAK gene, but the full-length natures of only three of them have been determined.

Specifications

• Antigen: Phospho-FAK (Tyr576, Tyr577)
• Applications: Western Blot, Immunocytochemistry
• Classification: Polyclonal
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.4
• Gene: PTK2
• Gene Accession No.: O35346, P34152, Q05397
• Gene Alias: CG10023; CG10023-PA; CG10023-PB; CG10023-PC; CG10023-PD; CG10023-PF; CG10023-PG; CT28129; DFAK; DFak56; Dmel\CG10023; Dmel_CG10023; DmFAK; EC 2.7.10.2; Fadk; FADK 1; Fak; Fak1; Fak56; fak56D; FAK65D; Fak-PA; Fak-PB; Fak-PC; Fak-PD; Fak-PF; Fak-PG; FAK-related non-kinase polypeptide; focal adhesion kinase; Focal adhesion kinase 1; focal adhesion kinase homolog; focal adhesion kinase isoform FAK-Del33; focal adhesion kinase pp125FAK; focal adhesion kinase-related nonkinase; focal ashension kinase 1; FRNK; I79_019430; Kiaa4203; mKIAA4203; p125FAK; p41/p43FRNK; pFAK; pp125 PTK2; pp125FAK; PPP1R71; Protein phosphatase 1 regulatory subunit 71; protein phosphatase 1, regulatory subunit 71; protein tyrosine kinase 2; protein tyrosine kinase 2 L homeolog; protein-tyrosine kinase; protein-tyrosine kinase 2; PTK2; PTK2 protein tyrosine kinase 2; ptk2.L; tyrosine kinase; XELAEV_18032408mg; XPFAK
• Gene Symbols: PTK2
• Host Species: Rabbit
• Immunogen: Peptide sequence around phosphorylation site of tyrosine 576/tyrosine 577 (S-T-Y(p)-Y(p)-K-A) derived from Human FAK.
• Purification Method: Antigen affinity chromatography
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 14083, 25614, 5747
• Target Species: Human, Mouse, Rat
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG

Frequently Asked Questions (FAQs)

How does the phosphorylation of FAK affect its function?

Focal adhesion kinase (FAK) can be phosphorylated at different sites, this affects its functions as follows:
FAK [pSer722]: Activated in response to cell adhesion; thought to function in opposition to multiple tyrosine phosphorylation events on FAK.
FAK [pSer732]: This is the site phosphorylated by CDK5. The functional importance is unknown but may play a role in cell cycle regulation in the brain, in particular.
FAK [pSer843]: Activated via cell adhesion, and during mitosis it is thought to function in opposition to multiple tyrosine phosphorylation events on FAK.
FAK [pSer910]: Activated via cell adhesion, and during mitosis it is thought to function in opposition to multiple tyrosine phosphorylation events on FAK.
FAK [pTyr397]: Autophosphorylation site (initial activation step) allows Src & p85 subunit of PI3-kinase to bind, and activates cell migration and invasion.
FAK [pTyr407]: Site is activated by TGFbeta and the Epithelial-Mesenchyme Transition (EMT); unlike other sites on FAK, phosphorylation of Tyr407 is EGFR independent.
FAK [pTyr576]: This site is phosphorylated by Src, and is located in the kinase activation loop (YY 576/577 motif); these sites appear to be differentially regulated by stimuli.
FAK [pTyr577]: This site is phosphorylated by Src, and is located in kinase activation loop (YY 576/577 motif), these sites appear to be differentially regulated by stimuli.
FAK [pY861]: This is also a Src site (major), it promotes binding to the cytoplasmic tail of integrins.
FAK [pY925]: This is a Src site (weak), it is GRB2 binding site allowing activation of the Ras/Raf/MEK signaling pathway.