Phospho-gamma-H2AX (Ser139) Polyclonal Antibody, Bethyl Laboratories

Rabbit Polyclonal Antibody

Supplier:  Bethyl Laboratories, Inc IHC00059T

Catalog No. 50-157-1410

Description

The recommended shelf life for this product is 1 year from date of receipt. Based on 100% sequence identity, this antibody is predicted to react with Bovine, Dog, Rabbit, Guinea pig_10141, Pig, Monkey, Gorilla, Little brown bat, hirteen-lin

Histone H2A.X (H2AX) is a member of the histone H2A family which is one of the four core histones making up the nucleosome core particle. In eukaryotes, DNA double strand breaks (DSBs) have been shown to trigger the phosphorylation of serine 139 at the carboxy terminus of histone H2AX resulting in gamma-H2AX. The phosphorylation of H2AX can be detected by Western blotting or immunofluorescence, revealing the frequency of DSBs. The phosphatidylinositol 3-kinases have been implicated in H2AX phosphorylation, but it is unclear if ATM is the primary H2AX kinase or if other members of the family such as DNA-PK and ATR contribute in a similar manner. Structurally, H2A.x contains 143 amino acid residues. Histone H2A.X is considered a basal histone, being synthesized in G1 as well as in S-phase, and its mRNA contains polyA addition motifs and a polyA tail along with the conserved stem-loop and U7 binding sequences involved in the processing and stability of replication type histone mRNAs. There are two forms of Histone H2A.X mRNA, one about 1600 bases long and contains polyA; the other about 575 bases long, lacking polyA. The short form behaves as a replication type histone mRNA, while the longer behaves as a basal type histone mRNA. Histone H2A.X maps to the 11q23.2-q23.3 region of the human chromosome. Histone H2A.x contributes to histone-formation and therefore the structure of DNA. Histone H2A variant H2A.x specifically regulates the interaction of MDC1 (mediator of DNA damage checkpoint protein 1), a DNA repair protein to the sites of DNA damage.

Specifications

• Antigen: Phospho-gamma-H2AX (Ser139)
• Classification: Polyclonal
• Formulation: TBS with 0.1% BSA and 0.09% sodium azide
• Gene Accession No.: P16104, P27661
• Gene Symbols: H2afx
• Immunogen: Surrounding serine 139
• Quantity: 10 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG

• Applications: Immunocytochemistry, Immunohistochemistry
• Conjugate: Unconjugated
• Gene: H2AFX
• Gene Alias: H2A/X, H2AX, H2AX histone, histone H2AX
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 15270, 3014
• Content And Storage: 4° C
• Form: Liquid