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Phospho-gamma-H2AX (Ser139) Polyclonal Antibody, Bethyl Laboratories

Catalog No. 501571410
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50-157-1410 10 μL
50-157-1409 100 μL
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Catalog No. 50-157-1410 Supplier Bethyl Laboratories, Inc Supplier No. IHC00059T
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Description

Rabbit Polyclonal Antibody

The recommended shelf life for this product is 1 year from date of receipt. Based on 100% sequence identity, this antibody is predicted to react with Bovine, Dog, Rabbit, Guinea pig_10141, Pig, Monkey, Gorilla, Little brown bat, hirteen-lin

Histone H2A.X (H2AX) is a member of the histone H2A family which is one of the four core histones making up the nucleosome core particle. In eukaryotes, DNA double strand breaks (DSBs) have been shown to trigger the phosphorylation of serine 139 at the carboxy terminus of histone H2AX resulting in gamma-H2AX. The phosphorylation of H2AX can be detected by Western blotting or immunofluorescence, revealing the frequency of DSBs. The phosphatidylinositol 3-kinases have been implicated in H2AX phosphorylation, but it is unclear if ATM is the primary H2AX kinase or if other members of the family such as DNA-PK and ATR contribute in a similar manner. Structurally, H2A.x contains 143 amino acid residues. Histone H2A.X is considered a basal histone, being synthesized in G1 as well as in S-phase, and its mRNA contains polyA addition motifs and a polyA tail along with the conserved stem-loop and U7 binding sequences involved in the processing and stability of replication type histone mRNAs. There are two forms of Histone H2A.X mRNA, one about 1600 bases long and contains polyA; the other about 575 bases long, lacking polyA. The short form behaves as a replication type histone mRNA, while the longer behaves as a basal type histone mRNA. Histone H2A.X maps to the 11q23.2-q23.3 region of the human chromosome. Histone H2A.x contributes to histone-formation and therefore the structure of DNA. Histone H2A variant H2A.x specifically regulates the interaction of MDC1 (mediator of DNA damage checkpoint protein 1), a DNA repair protein to the sites of DNA damage.

Specifications

Antigen Phospho-gamma-H2AX (Ser139)
Applications Immunocytochemistry, Immunohistochemistry
Classification Polyclonal
Conjugate Unconjugated
Formulation TBS with 0.1% BSA and 0.09% sodium azide
Gene H2AFX
Gene Accession No. P16104, P27661
Gene Alias H2A/X, H2AX, H2AX histone, histone H2AX
Gene Symbols H2afx
Host Species Rabbit
Immunogen Surrounding serine 139
Purification Method Antigen affinity chromatography
Quantity 10 μL
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 15270, 3014
Target Species Human, Mouse
Content And Storage 4° C
Product Type Antibody
Form Liquid
Isotype IgG
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