Invitrogen™ Phospho-GSK3 alpha/beta (Tyr279, Tyr216) Monoclonal Antibody (M132)

Catalog No.	Quantity
PIMA547628	100 μL

Catalog No. PIMA547628 Supplier Invitrogen™ Supplier No. MA547628

Description

Mouse Monoclonal Antibody

The antibody detects 46/50 kDa* proteins corresponding to the apparent molecular mass of GSK-3beta and GSK-3alpha on SDS-PAGE immunoblots of pervanadate treated rabbit fibroblasts, as well as treated human SKN-SH and A431 cells.

Glycogen synthase kinase-3 (GSK3) is a protein kinase that was originally identified as a regulator of glycogen synthase, a key enzyme in glycogen metabolism. Since then, it has been shown to be involved in the regulation of a diverse array of cellular functions, including protein synthesis, cell proliferation, cell differentiation, microtubule assembly/disassembly, and apoptosis. GSK3's substrate specificity is unique in that phosphorylation of the substrate only occurs if a phosphoserine or phosphotyrosine is present - four residues C-terminal to the site of GSK phosphorylation. There exists two isoforms of GSK3, GSK3 alpha and GSK3 beta, and they are strictly regulated via phosphorylation. Phosphorylation of GSK3 beta on Ser9 (Ser21 in GSK3alpha) by protein kinase B (PKB) causes its inactivation and is the primary mechanism responsible for growth factor inhibition of this kinase. Activation of GSK3 beta is dependent upon the phosphorylation of Tyr216 (Tyr279 in GSK3 alpha). Upon activation, it has been shown to phosphorylate a number of different cellular proteins, including p53, c-Myc, c-Jun, heat shock factor-1 (HSF-1), and cyclin D1. GSK3 beta also has been shown to phosphorylate aberrant sites on the microtubule associated protein tau, which is critical for the progression of Alzheimer's disease.

Specifications

• Antigen: Phospho-GSK3 alpha/beta (Tyr279, Tyr216)
• Applications: Western Blot
• Classification: Monoclonal
• Clone: M132
• Concentration: 0.25 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 1mg/mL BSA, 50% glycerol and 0.05% sodium azide
• Gene: GSK3A
• Gene Accession No.: P18265, P18266, P49840, P49841, Q2NL51, Q9WV60
• Gene Alias: 2700086H06Rik; 7330414F15Rik; 8430431H08Rik; C86142; FA; factor A; glycogen synthase kinase 3 alpha; glycogen synthase kinase 3 beta; glycogen synthase kinase-3 alpha; Glycogen synthase kinase-3 beta; GSK3; GSK-3; GSK-3 alpha; GSK-3 beta; Gsk3a; gsk-3-alpha; Gsk3b; GSK-3beta; GSK3beta isoform; serine/threonine-protein kinase GSK3A; Serine/threonine-protein kinase GSK3B
• Gene Symbols: GSK3A, GSK3B
• Host Species: Mouse
• Immunogen: Clone M132 was generated from a phospho-GSK-3beta (Tyr-216) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 216 of human GSK-3beta. This peptide sequence is also found in GSK-3alpha (Tyr-279) and is highly conserved in GSK-3 genes in rat and mouse.
• Purification Method: Protein A
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 2931, 2932, 50686, 56637, 606496, 84027
• Target Species: Human, Mouse, Rat
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1