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Phospho-Histone H2A.X (Ser139) Mouse anti-Human, Mouse, Clone: CR55T33, eBioscience™ Invitrogen™

Mouse Monoclonal Antibody

Manufacturer:  LIFE TECHNOLOGIES 14986537


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Catalog No. 14-986-537



The CR55T33 MAb recognizes phosphorylated serine 139 of human and mouse H2AX. H2AX is a member of the H2A histone family that complex with DNA and other histones to form the repeating nucleosome units characteristic of eukaryotic chromatin. Nucleosomes consist of approximately 147 base pairs of DNA wrapped around an octamer of histones composed of two each of the four histone proteins: H2A, H2B, H3 and H4. After induction of DNA damage such as double-strand breaks by irradiation, genotoxic stresses, replication errors or gene recombination, PI3K-like kinases (e.g., ataxia telangiectasia mutated (ATM), ataxia telangiectasia Rad-3-related (ATR), and DNA-dependent protein kinase (DNA-PK) are activated to phosphorylate serine 139 in H2AX. This early phosphorylation event plays a critical role in recruiting proteins involved in DNA repair. The MAb CR55T33 recognizes a single band of approximately 15 kDa on reduced cell lysates from Jurkat cells stimulated with etoposide.

Phospho-Histone H2A.X (Ser139) Monoclonal antibody specifically detects Phospho-Histone H2A.X (Ser139) in Human, Mouse samples. It is validated for Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Western Blot


Phospho-Histone H2A.X (Ser139)
0.5 mg/mL
PBS with 0.09% sodium azide; pH 7.2
P16104, P27661
2 mg
Human, Mouse
IgG1 κ
Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Western Blot
AW228881; gamma H2AX; gammaH2ax; H2A histone family member X; H2A histone family, member X; H2A.X; H2a/x; h2afx; H2ax; H2AX histone; Hist5-2ax; histone 5 protein 2ax; histone H2A.x; Histone H2a/x; Histone H2AX; RGD1566119; similar to H2A histone family, member X; zgc:56329
Affinity chromatography
15270, 3014
4° C


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