Invitrogen™ Phospho-Histone H2A.X (Ser139) Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA577995

Catalog No. PIPA577995

Description

Positive Control: HCT116, HCT116 (30 μM cisplatin treatment for 24 hr), HCT116 (UVB treatment 50mJ for 2hr), HCT116 (UVB treatment 50mJ for 6hr), NIH-3T3 (30μM cisplatin treatment for 24hr), PC-12 (30uM cisplatin treatment for 24hr) Predicted Reactivity: Rhesus Monkey (100%), Bovine (100%) Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Histone H2A.X (H2AX) is a member of the histone H2A family which is one of the four core histones making up the nucleosome core particle. In eukaryotes, DNA double strand breaks (DSBs) have been shown to trigger the phosphorylation of serine 139 at the carboxy terminus of histone H2AX resulting in gamma-H2AX. The phosphorylation of H2AX can be detected by Western blotting or immunofluorescence, revealing the frequency of DSBs. The phosphatidylinositol 3-kinases have been implicated in H2AX phosphorylation, but it is unclear if ATM is the primary H2AX kinase or if other members of the family such as DNA-PK and ATR contribute in a similar manner. Structurally, H2A.x contains 143 amino acid residues. Histone H2A.X is considered a basal histone, being synthesized in G1 as well as in S-phase, and its mRNA contains polyA addition motifs and a polyA tail along with the conserved stem-loop and U7 binding sequences involved in the processing and stability of replication type histone mRNAs. There are two forms of Histone H2A.X mRNA, one about 1600 bases long and contains polyA; the other about 575 bases long, lacking polyA. The short form behaves as a replication type histone mRNA, while the longer behaves as a basal type histone mRNA. Histone H2A.X maps to the 11q23.2-q23.3 region of the human chromosome. Histone H2A.x contributes to histone-formation and therefore the structure of DNA. Histone H2A variant H2A.x specifically regulates the interaction of MDC1 (mediator of DNA damage checkpoint protein 1), a DNA repair protein to the sites of DNA damage.

Specifications

• Antigen: Phospho-Histone H2A.X (Ser139)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: H2AX
• Gene Alias: AW228881; gamma H2AX; gammaH2ax; H2A histone family member X; H2A histone family, member X; H2A.X; H2A.X variant histone; H2A/X; h2afx; H2ax; H2AX histone; Hist5-2ax; histone 5 protein 2ax; histone H2A.x; Histone H2a/x; Histone H2AX; RGD1566119; similar to H2A histone family, member X; zgc:56329
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 15270, 3014, 394048, 500987
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Frozen), Immunohistochemistry (Free Floating), Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Concentration: 0.25 mg/mL
• Formulation: PBS with 1% BSA, 20% glycerol and 0.025% ProClin 300; pH 7
• Gene Accession No.: P16104, P27661, Q7ZUY3
• Gene Symbols: H2AX
• Immunogen: Carrier-protein conjugated synthetic peptide surrounding phospho Ser139 of human Histone H2A.X. The exact sequence is proprietary.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat, Zebrafish
• Product Type: Antibody
• Isotype: IgG