Invitrogen™ Phospho-INSR (Tyr1334) Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ 44809G

Catalog No. 44-809-G

Description

This antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Insulin Receptor. The final product is generated by affinity chromatography using a Insulin Receptor-derived peptide that is phosphorylated at tyrosine 1334. This antibody recognizes human insulin receptor phosphorylated at tyrosine 1334 as well as the human IR Isoform B phosphorylated at tyrosine 1332. Western blot positive control used was insulin-treated CHO-T cells expressing Human Insulin Receptor.

INSR is a receptor tyrosine kinase. Binding of insulin to the extracellular domain of INSR stimulates glucose uptake. INSR and IGF-1 receptors share major structural and functional similarity. The earliest cellular response to insulin stimulation is autophosphorylation of tyrosine in INSR. In humans, the INSR gene is located on chromosome 19. Defects in INSR are the cause of various insulin resistance syndromes and IGF-1R defects may also cause some forms of growth retardation.

Specifications

• Antigen: Phospho-INSR (Tyr1334)
• Classification: Polyclonal
• Formulation: Dulbecco's PBS with 1mg/mL BSA and 0.05% sodium azide; pH 7.3
• Gene Accession No.: P06213, P15127, P15208
• Gene Symbols: INSR
• Immunogen: The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human Insulin Receptor that contains tyrosine 1334.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG

• Applications: Western Blot, Immunocytochemistry
• Conjugate: Unconjugated
• Gene: INSR
• Gene Alias: 4932439J01Rik; CD 220; CD220; CD220 antigen; CD220 beta; CD221; D630014A15Rik; HHF 5; HHF5; IGF-I receptor; INSR; Insulin receptor; insulin receptor preproprotein; Insulin receptor subunit alpha; Insulin receptor subunit beta; IR; IR 1; IR beta; IR1; IR-1; IR-A; IR-B
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 16337, 24954, 3643
• Content And Storage: -20°C
• Form: Liquid