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Invitrogen™ Phospho-IRS1 (Ser307) Polyclonal Antibody
Rabbit Polyclonal Antibody
Supplier: Invitrogen™ PA11054
Description
PA1-1054 detects phosphorylated IRS-1 (Ser307) in mouse and human cells. PA1-1054 has been successfully used in Western Blot procedures. By WB, this antibody detects an ~170 kDa protein representing phospho-IRS-1 (Ser307) from 3T3 L1 cells treated with insulin for 10 minutes. The PA1-1054 immunogen is a synthetic phosphopeptide corresponding to residues E(301) S I T A T (pS) P A S M V G G K(315) of human IRS-1. This peptide is 100% conserved in rats and mice. This peptide (Cat. #PEP-184) is available for use in neutralization and control experiments.
IRS-1, a major substrate of the insulin receptor, is phosphorylated in response to stimulation of cells by insulin, insulin-like growth factor 1 (IGF-1) and interleukin 4 (IL-4). IRS-1 is phosphorylated on serine, threonine and tyrosine residues in a variety of tissues. An insulin-sensitive serine/threonine kinase casein kinase II mediates a portion of the insulin-stimulated serine/threonine phosphorylation of overexpressed IRS-1 in vivo. Thr 502 is identified as the major casein kinase II-catalyzed phosphorylation site in rat IRS-1, and Ser 99 is an additional phosphorylation site catalyzed by casein kinase II. Thus, casein kinase II-catalyzed phosphorylation of IRS-1 may be a component of the intracellular insulin signaling cascade. IRS-1 contains three putative binding sites for 14-3-3 (Ser 270, Ser 374 and Ser 641) and the motif around Ser 270 is located in the phosphortyrosine binding domain of IRS-1, which is responsible for the interaction with the insulin receptor.
Specifications
Phospho-IRS1 (Ser307) | |
Polyclonal | |
Unconjugated | |
IRS1 | |
G972R; HIRS-1; Insulin receptor substrate 1; IRS1; IRS-1; IRS1IRM; pp185 | |
Rabbit | |
Antigen Affinity Chromatography | |
RUO | |
16367, 3667 | |
-20°C, Avoid Freeze/Thaw Cycles | |
Liquid |
Western Blot, Immunocytochemistry | |
1 mg/mL | |
PBS with 1mg/mL BSA and 0.05% sodium azide | |
P35568, P35569 | |
IRS1 | |
Synthetic phosphopeptide corresponding to residues E(301) S I T A T (pS) P A S M V G G K(315) of human IRS-1. | |
100 μg | |
Primary | |
Human, Mouse | |
Antibody | |
IgG |
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