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Invitrogen™ Phospho-IRS1 (Tyr612) Polyclonal Antibody
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Catalog No. 44816G
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44-816-G 100 μL
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Catalog No. 44-816-G Supplier Invitrogen™ Supplier No. 44816G
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Description

Rabbit Polyclonal Antibody

On western blotting this antibody detects a protein with the apparent MW of 165 kDa. Positive controls used were Insulin (50 nM for 5 minutes) or TPA (100 ng/mL for 30 min) treated human embryonic kidney cells (293T) or Chinese Hamster Ovary cells (CHO-T) transiently transfected with a plasmid encoding human IRS1.

IRS-1, a major substrate of the insulin receptor, is phosphorylated in response to stimulation of cells by insulin, insulin-like growth factor 1 (IGF-1) and interleukin 4 (IL-4). IRS-1 is phosphorylated on serine, threonine and tyrosine residues in a variety of tissues. An insulin-sensitive serine/threonine kinase casein kinase II mediates a portion of the insulin-stimulated serine/threonine phosphorylation of overexpressed IRS-1 in vivo. Thr 502 is identified as the major casein kinase II-catalyzed phosphorylation site in rat IRS-1, and Ser 99 is an additional phosphorylation site catalyzed by casein kinase II. Thus, casein kinase II-catalyzed phosphorylation of IRS-1 may be a component of the intracellular insulin signaling cascade. IRS-1 contains three putative binding sites for 14-3-3 (Ser 270, Ser 374 and Ser 641) and the motif around Ser 270 is located in the phosphortyrosine binding domain of IRS-1, which is responsible for the interaction with the insulin receptor.
TRUSTED_SUSTAINABILITY

Specifications

Antigen Phospho-IRS1 (Tyr612)
Applications Immunohistochemistry (Paraffin), Western Blot
Classification Polyclonal
Conjugate Unconjugated
Formulation Dulbecco's PBS with 1mg/mL BSA and 0.05% sodium azide; pH 7.3
Gene IRS1
Gene Accession No. P35568
Gene Alias G972R; HIRS-1; Insulin receptor substrate 1; IRS1; IRS-1; IRS1IRM; pp185
Gene Symbols IRS1
Host Species Rabbit
Immunogen The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human IRS-1 that contains tyrosine 612. The sequence is conserved in mouse, rat and chicken.
Purification Method Antigen affinity chromatography
Quantity 100 μL
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 3667
Target Species Human
Content And Storage -20°C
Product Type Antibody
Form Liquid
Isotype IgG
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Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov
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