MilliporeSigma™ phospho-JAK1 (Tyr1022/1023), Rabbit, Unlabeled, Polyclonal,

Catalog No.	Quantity	Conjugate
07849I	100 μL	Unconjugated

Catalog No. 07849I Supplier MilliporeSigma™ Supplier No. 07849I

Description

Rabbit Polyclonal Antibody

Merlin (UniProt P35240; also known as Moesin-ezrin-radixin-like protein, Neurofibromin-2, Schwannomerlin, Schwannomin) is encoded by the NF2 (also known as SCH) gene (Gene ID 4771) in human. Originally identified as a tumor suppressor gene in the familial Neurofibromatosis type 2 (NF2) cancer patients, NF2 encodes the FERM (Ezrin, Radixin, Moesin) domain-containing merlin that plays a negative regulating role against cell growth and proliferation by functioning as an upstream activator of the Hippo pathway. Merlin directly activates Mst1/2 and recruits Lats1/2 to the plasma membrane for phosphorylation by Mst1/2. The phosphorylated Lats1/2 in turn inhibits YAP/TAZ transcription activity by phosphorylating YAP/TAZ and causes its cytoplasmic sequestration. Merlin also translocates to the nucleus, where it inhibits E3 ubiquitin ligase CRL4(DCAF1), thereby preventing it from ubiquitinating and downregulating Lats1/2. In its inactive state, the C-terminal tail domain (CTD; a.a. 507-595) of merlin is bound intramolecularly to its FERM domain (a.a. 19-313). Angiomotin (AMOT) induces merlin activation by binding to an elongated helical fragment within merlin CTD and subsequently releases the auto-inhibited conformation of Merlin. Merlin CTD can also undergo phosphorylation on Ser518 by p21-activated kinase (PAK) or cyclic AMP-dependent protein kinase (PKA), resulting in a weakened AMOT binding and a stabilization of merlin in its auto-inhibited state.

Specifications

• Antigen: phospho-JAK1 (Tyr1022/1023)
• Applications: Western Blot
• Classification: Polyclonal
• Concentration: Please refer to lot specific datasheet.
• Conjugate: Unconjugated
• Formulation: Purified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
• Gene: JAK1, JAK1A, JAK1B
• Gene Accession No.: P23458
• Host Species: Rabbit
• Immunogen: KLH-conjugated phosphopeptide corresponding to the epitope reqeion sequence containing the two phosphorylated target tyrosine residues.
• Purification Method: Affinity Purified
• Quantity: 100 μL
• Regulatory Status: RUO
• Research Discipline: Signaling
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_002218
• Test Specificity: The Tyr1022/1023 designation is according to the JAK1 sequence originally published by Wilks, A.F., et al. (1991). Mol. Cell. Biol. 11(4):2057-2065 (PMID 1848670; NCBI accession: AAA36527). Numbering of the target pTyr residues varies depending on the species and source of the sequence. For example, target pTyr residues this rabbit polyclonal detects correspond to pTyr1034/pTyr1035 and pTyr1033/pTyr1034, respectively, of the human and mouse JAK1 sequence reported by UniProt (P23458 and P52332, respectively), or pTyr1033/pTyr1034 of bovine, canine, and rat JAK1 (NP_001193463 and NP_445918.1), pTyr1021/pTyr1022 of porcine JAK1 (NP_999279).
• Target Species: Human
• Content And Storage: Stable for 1 year at 2°-8°C from date of receipt.

For Research Use Only. Not for use in diagnostic procedures.