Invitrogen™ Phospho-JAK1 (Tyr1034, Tyr1035) Recombinant Rabbit Monoclonal Antibody (59H4L5)

Catalog No.	Quantity
PI700028	100 μg

Catalog No. PI700028 Supplier Invitrogen™ Supplier No. 700028

Description

Rabbit Recombinant Monoclonal Antibody

This antibody is predicted to react with mouse, rat, chimpanzee, Rhesus monkey, bovine, canine, porcine, equine, Xenopus and zebrafish based on sequence homology.Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.This product was formerly called Phospho-JAK1 (Tyr1022, Tyr1023) because Human Jak1 residues Tyr1034 and Tyr1035 have been historically referenced as Tyr1022 and Tyr1023.

Janus kinase 1 (JAK1), is a protein tyrosine kinase that is involved in the interferon-alpha/beta and -gamma signal transduction pathways. Immunological stimuli, such as interferons and cytokines, induce recruitment of Stat transcription factors to cytokine receptor-associated JAK1. JAK1 then phosphorylates proximal Stat factors, which subsequently dimerize, translocate to the nucleus and bind to cis elements upstream of target gene promoters to regulate transcription. Upon ligand binding, JAK1 undergoes tyrosine phosphorylation and catalytic activation in an interdependent manner. Phosphorylation of tyrosine residues at positions 1022 and 1023 is believed to function in the activation of catalytic events.

Specifications

• Antigen: Phospho-JAK1 (Tyr1034, Tyr1035)
• Applications: ChIP Assay, Western Blot, Immunocytochemistry
• Classification: Recombinant Monoclonal
• Clone: 59H4L5
• Concentration: 0.5 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 0.09% sodium azide
• Gene: JAK1
• Gene Accession No.: P23458, P52332
• Gene Alias: AA960307; BAP004; C130039L05Rik; EC 2.7.10.2; Jak1; JAK-1; JAK1A; JAK1B; Janus kinase 1; Janus protein tyrosine kinase 1; JTK3; kinase Jak1; Tyrosine-protein kinase JAK1
• Gene Symbols: JAK1
• Host Species: Rabbit
• Immunogen: A peptide corresponding to amino acids 1030-1039 of P23458. This site is commonly referred to as pY1022/pY1023 in the literature.
• Purification Method: Protein A
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 16451, 3716
• Target Species: Human, Mouse
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG