Invitrogen™ Phospho-Stat4 (Tyr693) Recombinant Rabbit Monoclonal Antibody (Stat4Y693-F6)

Catalog No.	Quantity
PIMA537080	20 μL

Catalog No. PIMA537080 Supplier Invitrogen™ Supplier No. MA537080

Description

Rabbit Recombinant Monoclonal Antibody

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

STAT4 was originally identified using degenerate primers complementary to sequences encoding conserved regions of other STAT proteins. The STAT4 protein is most similar to STAT1 (52%) and STAT3 (47%). Functionally, STAT4 is similar to other STAT family members in that it can be tyrosine phosphorylated by Jak1 or Jak2. STAT4 forms homodimers and heterodimers with related STAT family members. Tyrosine phosphorylated STAT4 can bind the IFN-gamma activated site (GAS). Serine phosphorylation of STAT is also required for maximal transcriptional activity. STAT4 expression is restricted to the thymus, spleen and testis. Until recently the cytokine(s) responsible for activation of STAT4 had not been identified. STAT4 is now known to be activated by the cytokine interleukin 12 (IL-12). IL-12 is required for the T-cell independent induction of IFN-gamma which is a key step in the initial suppression of bacterial and parasitic infections. In addition, IL-12 is required for the development of a Th1 response which is necessary for effective host defense against intracellular pathogens. STAT4-deficient mice display impaired IL-12 development of Th1 cells and enhanced development of Th2 cells. A recent study in mouse has shown that in response to viral infection IFN-a/b activation of STAT4 is required for IFN-g production.

Specifications

• Antigen: Phospho-Stat4 (Tyr693)
• Applications: Flow Cytometry
• Classification: Recombinant Monoclonal
• Clone: Stat4Y693-F6
• Concentration: 0.5 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 0.1% BSA, 50% glycerol and 0.02% sodium azide; pH 7.4
• Gene: STAT4
• Gene Accession No.: P42228, Q14765
• Gene Alias: CIS4; HSPC060; OTTHUMP00000163553; Signal transducer and activator of transcription 4; SLEB11; SOCS4; SOCS6; SSI4; STAI4; STAT; Stat4; STATI4
• Gene Symbols: STAT4
• Host Species: Rabbit
• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Tyr693 of human phospho Stat4.
• Purification Method: Protein A
• Quantity: 20 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 20849, 6775
• Target Species: Human, Mouse
• Content And Storage: -20°C
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG κ