Invitrogen™ Phospho-MST1 (STK4) (Thr183) Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA540177

Catalog No. PA540177

Description

This antibody is predicted to react with mouse based on sequence homology. The antibody detects endogenous levels of Mst1 only when phosphorylated at threonine 183.

STK4 (MST1) is a serine/threonine kinase which may be involved in apoptosis. It is possible that this protein induces the chromatin condensation observed in this process. STK4 is a stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. It is a key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. STK3/MST2 and STK4/MST1 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation.

Specifications

• Antigen: Phospho-MST1 (STK4) (Thr183)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Stk4
• Gene Alias: AI447339; AU020804; EC 2.7.11.1; Kas-2; kinase MST1; kinase responsive to stress 2; KRS2; Mammalian STE20-like protein kinase 1; mammalian sterile 20-like 1; MST1; MST-1; MST1/C; MST1/N; serine/threonine kinase 4; serine/threonine-protein kinase 4; Serine/threonine-protein kinase 4 18kDa subunit; Serine/threonine-protein kinase 4 37kDa subunit; Serine/threonine-protein kinase Krs-2; STE20-like kinase MST1; sterile 20-like kinase 1; STK4; TIIAC; Yeast Sps1/Ste20-related kinase 3; Ysk3
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 58231, 6789
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.4
• Gene Accession No.: Q13043, Q9JI11
• Gene Symbols: Stk4
• Immunogen: Peptide sequence around phosphorylation site of threonine 183 (R-N-T(p)-V-I) derived from Human Mst1.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG