Invitrogen™ Phospho-p38 MAPK (Thr180, Tyr182) Polyclonal Antibody

Catalog No.	Quantity
44-684-G	100 μL

Catalog No. 44-684-G Supplier Invitrogen™ Supplier No. 44684G

Description

Rabbit Polyclonal Antibody

This antibody reacts with human and rat (100% homologous) p38 alpha, beta, and gamma. HEK 293 cells +/- UV irradiation treatment and PC12 cells +/- Sorbitol were used as positive controls. Purified from rabbit serum by sequential epitope-specific chromatography, this product contains enough material for 10 mini-blots. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated p38. The final product is generated by affinity chromatography using a p38-derived peptide that is phosphorylated at threonine 180 and tyrosine 182.

MAPK14 (p38 alpha) is a member of the stress-activated protein kinase class of MAPKs. The p38 MAPK cascade regulates a variety of cellular responses to stress, inflammation, and other signals. p38 MAPK is relatively inactive in the nonphosphorylated form and becomes rapidly activated by dual phosphorylation of a Thr-Gly-Tyr motifs. There are four isoforms of p38 MAPK, which differ in their tissue expression and affinity for upstream activators and downstream effectors. When cells are exposed to tumor necrosis factor, interleukin-1, heat shock, or other activating stimuli, activation of MAPK kinase-3 occurs by phosphorylation. Activated MAPK kinase-3/6 phosphorylate each residue of Thr180 and Tyr182 in p38 MAPK. Phospho-p38 MAPK activates ATF-2, CHOP-1, MEF-2 and other transcription factors through phosphorylation.

Specifications

• Antigen: Phospho-p38 MAPK (Thr180, Tyr182)
• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Classification: Polyclonal
• Conjugate: Unconjugated
• Formulation: Dulbecco's PBS with 1mg/mL BSA and 0.05% sodium azide; pH 7.3
• Gene: MAPK14
• Gene Accession No.: P70618, Q16539
• Gene Alias: 186F5S; anon-sts23; BG:DS00797.3; CG7393; CG7393-PA; CRK1; CSAID-binding protein; Csaids binding protein; CSBP; Csbp1; Csbp2; CSPB1; cytokine suppressive anti-inflammatory drug binding protein; cytokine suppressive anti-inflammatory drug binding protein 1; cytokine suppressive anti-inflammatory drug-binding protein; cytokine-supressive anti-inflammatory drug binding protein; Dm p38b; Dmel\CG7393; Dmel_CG7393; Dmp38b; Dp38; D-p38; D-p38 MAPK; Dp38b; D-p38b; EC 2.7.11.24; ERK1; ERT2; ESTS:186F5S; etID18993.8; Exip; fk28c03; hm:zeh1243; Hog; I79_016605; LD24658p; MAP 14; MAP kinase 1; MAP kinase 11; MAP kinase 14; MAP kinase 14A; MAP kinase 2; MAP kinase Mxi2; MAP kinase p38; MAP kinase p38 alpha; MAP kinase p38 beta; MAP kinase p38a; MAP kinase p38alpha; MAP kinase p38b; MAPK; MAPK 11; MAPK 14; MAPK 14A; MAPK1 antibody; Mapk11; Mapk14; mapk14a; MAX-interacting protein 2; mitogen activated protein kinase 11; mitogen activated protein kinase 14; mitogen-activated protein kinase 11; Mitogen-activated protein kinase 14; mitogen-activated protein kinase 14a; Mitogen-activated protein kinase p38 alpha; mitogen-activated protein kinase p38 beta; mitogen-activated protein kinase p38-2; mitogen-activated protein kinase p38a; Mitogen-activated protein kinase p38b; MK14; Mpk34C; Mxi2; OTTHUMP00000216563; OTTHUMP00000216566; P38; p38 alpha; p38 alpha/Mxi2; p38 beta; p38 MAP kinase; p38 MAP kinase alpha; p38 MAPK; p38 mitogen activated protein kinase; p38 mitogen-activated protein kinase; p38-2; p38a; p38ALPHA; p38-alpha; p38alpha Exip; P38B; p38b MAP kinase; p38beta; p38beta2; p38b-PA; p38Hog; p38Kb; p38MAPK; PRKM11; Prkm14; Prkm15; protein kinase, mitogen activated kinase, 11, p38beta; reactive kinase; RK; RP1-179N16.5; Sapk2; Sapk2A; SAPK2b; stress-activated p38b MAP kinase; stress-activated protein kinase 2A; Stress-activated protein kinase 2b; stress-activated protein kinase-2; stress-activated protein kinase-2b; tRNA synthetase cofactor p38; wu:fk28c03; zeh1243; zp38a
• Gene Symbols: MAPK14
• Host Species: Rabbit
• Immunogen: The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human p38 that contains threonine 180 and tyrosine 182. This region is conserved among many species including mouse, rat, dog, monkey and carp.
• Purification Method: Antigen affinity chromatography
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 1432, 81649
• Target Species: Human, Rat
• Content And Storage: -20°C
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG