Invitrogen™ Phospho-PAK4/6/7 (Ser474) Recombinant Rabbit Monoclonal Antibody (1H7L3)

Catalog No.	Quantity
PI702663	100 μg

Catalog No. PI702663 Supplier Invitrogen™ Supplier No. 702663

Description

Rabbit Recombinant Monoclonal Antibody

This antibody is predicted to react with Monkey, Horse, Rat Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

PAK4 is highly expressed in prostate, testis and colon. PAK4 interacts tightly with GTP-bound but not GDP-bound CDC42 and weakly with RAC. PAK4 phosphorylates and autophosphorylates and also activates the JNK pathway. PAK5, a member of the PAK family of protein kinases contains a CDC42/Rac1 interactive binding (CRIB) motif at the N-terminus and a Ste20-like kinase domain at the C-terminus. PAK5 preferentially binds to CDC42 in the presence of GTP and the CRIB motif is essential for this interaction. PAK6 protein cotranslocates into the nucleus with androgen receptor, which is a steroid hormone-dependent transcription factor that is important for male sexual differentiation and development, in response to androgen. PAK6 was initially identified as an androgen receptor in a yeast two hybrid screen and was found to be highly expressed in testis and prostate tissues. Later experiments have shown it to be activated by MAP kinase 6 and p38 MAP kinase, suggesting that PAK6 may play a role in the cellular response to stress-related signals.

Specifications

• Antigen: Phospho-PAK4/6/7 (Ser474)
• Applications: Western Blot, Immunocytochemistry
• Classification: Recombinant Monoclonal
• Clone: 1H7L3
• Concentration: 0.5 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 0.09% sodium azide; pH 7.2-7.4
• Gene: Pak4
• Gene Accession No.: O96013, Q9NQU5, Q9P286
• Gene Alias: 2900083L08Rik; 4732456M09; 5730488L07Rik; 6430627N20; AW555722; KIAA1142; KIAA1264; mKIAA1142; p21 (CDKN1A)-activated kinase 4; p21 (CDKN1A)-activated kinase 6; p21 (CDKN1A)-activated kinase 7; p21 (RAC1) activated kinase 4; p21 (RAC1) activated kinase 5; p21 (RAC1) activated kinase 6; p21 (RAC1) activated kinase 7; p21 protein (Cdc42/Rac)-activated kinase 4; p21 protein (Cdc42/Rac)-activated kinase 6; p21 protein (Cdc42/Rac)-activated kinase 7; p21(CDKN1A)-activated kinase 4; p21(CDKN1A)-activated kinase 6; p21(CDKN1A)-activated kinase 7; p21-activated kinase 4; p21-activated kinase 5; p21-activated kinase 6; p21-activated kinase 7; p21CDKN1A-activated kinase 7; PAK 4/5/6; PAK 4/6/7; PAK4; PAK-4; PAK4/5/6; PAK5; PAK-5; PAK6; PAK-6; Pak7; PAK-7; protein kinase PAK5; protein kinase related to S. cerevisiae STE20, effector for Cdc42Hs; serine/threonine-protein kinase PAK 4; Serine/threonine-protein kinase PAK 5; serine/threonine-protein kinase PAK 6; serine/threonine-protein kinase PAK 6-like; serine/threonine-protein kinase PAK 7; serine/threonine-protein kinase PAK7
• Gene Symbols: Pak4, PAK5, PAK6
• Host Species: Rabbit
• Immunogen: Peptide corresponding to human Phospho-PAK4/PAK5/PAK6 (Ser474) (aa472-aa480).
• Purification Method: Protein A
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 10298, 56924, 57144
• Target Species: Human
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov