Invitrogen™ Phospho-PDGFRA (Tyr754) Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ 441008G

Catalog No. 44-100-8G

Description

The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated PDGFRA. The final product is generated by affinity chromatography using a PDGFRA-derived peptide phosphorylated at tyrosine 754. Human, rat (100% homologous), and frog (92%) PDGFRA have not been tested, but are expected to react. PDGFRB (50%) has not been tested, but is not expected to react. Positive controls used with this antibody in western blotting were NIH3T3 cells +/- PDGF.

PDGFRA is a cell surface receptor tyrosine kinase which binds members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies in knockout mice, where homozygosity is lethal, indicate that the alpha form of the platelet-derived growth factor receptor is particularly important for kidney development since mice heterozygous for the receptor exhibit defective kidney phenotypes.

Specifications

• Antigen: Phospho-PDGFRA (Tyr754)
• Classification: Polyclonal
• Formulation: Dulbecco's PBS with 1mg/mL BSA and 0.05% sodium azide; pH 7.3
• Gene Accession No.: P16234, P20786, P26618
• Gene Symbols: PDGFRA
• Immunogen: The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human PDGFRa that contains tyrosine 754.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Conjugate: Unconjugated
• Gene: PDGFRA
• Gene Alias: AI115593; alpha platelet-derived growth factor receptor; Alpha platelet-derived growth factor receptor precursor (PDGF-R-alpha); alpha-type platelet-derived growth factor receptor; APDGFR; CD140 antigen-like family member A; CD140A; CD140a antigen; GAS9; MGC74795; OTTHUMP00000218656; PDGF A-chain; PDGF alpha chain; PDGF Receptor alpha; PDGF subunit A; PDGF-1; PDGFACE; PDGFR2; Pdgfr-2; PDGFRA; pdgfr-a; PDGFRA/BCR fusion; PDGF-R-alpha; PDGFR-alpha; platelet derived growth factor receptor alpha; platelet derived growth factor receptor, alpha polypeptide; platelet-derived growth factor alpha receptor; Platelet-derived growth factor receptor 2; platelet-derived growth factor receptor alpha; platelet-derived growth factor receptor, alpha polypeptide; rearranged-in-hypereosinophilia-platelet derived growth factor receptor alpha fusion protein; RHEPDGFRA
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 18595, 25267, 5156
• Content And Storage: -20°C
• Form: Liquid