Phospho-PDPK1 (Ser241) Rabbit anti-Human, Invitrogen™

Rabbit Recombinant Monoclonal Antibody

Manufacturer:  Invitrogen MA528025

Catalog No. PIMA528025

Description

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using Recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

PDK1 (3 Phosphoinositide Dependent Protein Kinase 1) phosphorylates AGC kinases. PDK1 activates conventional PKC and PKC zeta through phosphorylation of critical threonine residues in the activation loop. PDK1 also phosphorylates Protein Kinase B (PKB) at threonine 308 in the presence of hosphatidylinositol-3,4,5-trisphosphate. Active Akt inactivates Glycogen Synthase Kinase 3 (GSK3), eventually leading to the dephosphorylation and activation of glycogen synthase, and the stimulation of glycogen synthesis. Because of the role that PDK plays in insulin-induced glycogen synthesis and PKC activation, it is a potentially important target for metabolic drug research.

Specifications

• Antigen: Phospho-PDPK1 (Ser241)
• Classification: Recombinant Monoclonal
• Concentration: 0.5 mg/ml
• Formulation: PBS with 0.1% BSA, 50% glycerol and 0.02% sodium azide; pH 7.4
• Gene Accession No.: O15530
• Gene Symbols: PDPK1
• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Ser241 of human phospho PDPK1
• Purification Method: Protein A/G
• Regulatory Status: RUO
• Type: Antibody
• Monoclonal or Polyclonal: Monoclonal
• Target Species: Human

• Applications: Flow Cytometry
• Clone: PDPK1S241-F7
• Conjugate: Unconjugated
• Gene: PDPK1
• Format: Liquid
• Host Species: Rabbit
• Isotype: IgG1, kappa
• Quantity: 200 μl
• Storage Requirements: -20°C
• Primary or Secondary: Primary
• Gene ID (Entrez): 5170